siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells

Tanaka, Hiroki; Shirkoohi, Reza; Nakagawa, Koji; Qiao, Hongjiang; Fujita, Hisakazu; Okada, Futoshi; Hamada, Jun-ichi; Kuzumaki, Satoshi; Takimoto, Masato; Kuzumaki, Noboru

siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells

Tanaka, Hiroki; Shirkoohi, Reza; Nakagawa, Koji et al.

International Journal of Cancer, 2005, 118(7), 1680-1691

Permalink : https://hdl.handle.net/2115/715

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アクセス権	open access
URI	https://hdl.handle.net/2115/715
タイトル	siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells
著者	Tanaka, Hiroki ; Shirkoohi, Reza ; Nakagawa, Koji ; Qiao, Hongjiang ; Fujita, Hisakazu ; Okada, Futoshi ; Hamada, Jun-ichi ; Kuzumaki, Satoshi ; Takimoto, Masato ; Kuzumaki, Noboru
著者名 Tanaka, Hiroki
著者名 Shirkoohi, Reza
著者名 Nakagawa, Koji
著者名 Qiao, Hongjiang
著者名 Fujita, Hisakazu
著者名 Okada, Futoshi
著者名 Hamada, Jun-ichi
著者名 Kuzumaki, Satoshi 葛巻, 暹
著者名 Takimoto, Masato
著者名 Kuzumaki, Noboru
言語	英語
キーワード	actin-regulatory protein
	small interfering RNA
	EMT
発行日	2005-10-10
出版者	Wiley-Liss, Inc.
収録物名	International Journal of Cancer
巻(号)	118(7)
ページ	1680-1691
抄録	Epithelial-mesenchymal transition (EMT) describes a process occurring during development and oncogenesis by which epithelial cells obtain fibroblast-like properties and show reduced cell adhesion and increased motility. In this report we demonstrated typical EMT in human mammary epithelial MCF10A siRNA gelsolin knockdown cells. EMT was characterized by fibroblastic morphology, loss of contact inhibition and focus formation in monolayer growth, enhanced motility and invasiveness in vitro, increased actin filaments, overexpression of RAC, activation of both ERK and AKT, inactivation of GSK3, conversion of cadherin from the E- to N-type, and induction of the transcription factor Snail. These results suggested that gelsolin functions as a switch that controls E- and N-cadherin conversion via Snail,and demonstrated that its knockdown leads to EMT in human mammary epithelial cells and possibly to the development of human mammary tumors.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Copyright (c) 2005 Wiley-Liss, Inc
PISSN	0020-7136
関連情報	PMID 16217750
関連情報	URI http://www.interscience.wiley.com/
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1002/ijc.21559