open access

https://hdl.handle.net/2115/71573

Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency

英語

Salt tolerance

2017-10

Journal of Applied Phycology

29(5)

2617-2626

Seaweeds are believed to have developed unique mechanisms to maintain optimal cellular potassium and sodium concentrations in order to survive in the saline marine environment. To gain a molecular understanding of underlying potassium/sodium homeostasis in seaweeds, full-length cDNA libraries from the multiple stages in the life cycle, including gametophytes, conchosporangia and sporophytes of a marine red alga, Pyropia yezoensis, were constructed. A large portion of genes from each library through the life cycle was revealed to be functionally unknown reconfirming the uniqueness of P. yezoensis genes in terms of evolutionary lineage. Genes that could potentially contribute to potassium deficiency tolerance were selected from the potassium uptake defective Escherichia coli strain expressing gametophytes and conchosporangia libraries under the low potassium conditions. Of those, an ammonium transporter gene, PyAMT1, was demonstrated to enhance potassium deficiency tolerance effectively when expressed in the E. coli strain. Potential roles of PyAMT1 and other candidate components in this context are discussed.

学術雑誌論文

Accepted Manuscript

This is a post-peer-review, pre-copyedit version of an article published in Journal of Applied Phycology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10811-017-1196-1.

0921-8971

1573-5176