Isoflavones and their metabolites influence the milk component synthesis ability of mammary epithelial cells through prolactin/STAT5 signaling

Tsugami, Yusaku; Matsunaga, Kota; Suzuki, Takahiro et al.

Molecular nutrition & food research, 2017, 61(10), 1700156

Permalink : https://hdl.handle.net/2115/71595

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アクセス権
open access
URI
https://hdl.handle.net/2115/71595
タイトル
Isoflavones and their metabolites influence the milk component synthesis ability of mammary epithelial cells through prolactin/STAT5 signaling
著者
Tsugami, Yusaku ; Matsunaga, Kota ; Suzuki, Takahiro ; Nishimura, Takanori ; Kobayashi, Ken
著者名
Tsugami, Yusaku
著者名
Matsunaga, Kota
著者名
Suzuki, Takahiro
著者名
Nishimura, Takanori
e-Rad_Researcher
30449003
著者名
Kobayashi, Ken
言語
英語
キーワード
β-casein
Isoflavone
Mammary epithelial cell
Prolactin receptor
STAT5
発行日
2017-10
出版者
John Wiley & Sons
収録物名
Molecular nutrition & food research
巻(号)
61(10)
ページ
1700156
抄録
Scope: Isoflavones are a class of polyphonic compounds present in legumes and are called phytoestrogens because of their estrogen-like activity. Estrogen influences the behavior of mammary epithelial cells (MECs) during pregnancy and lactation. In this study, we investigated the direct influences of isoflavones and their metabolites in milk production ability of MECs. Methods and results: Mouse MECs were cultured with prolactin and dexamethasone (glucocorticoid analog) to induce milk production ability. Subsequently, lactating MECs were treated with each isoflavone. Coumestrol, biochanin A, genistein, and formononetin decreased the intracellular and secreted beta-casein. On the other hand, p-ethylphenol, daidzein, and equol did not significantly influence beta-casein production at any concentration. Coumestrol, biochanin A and genistein down-regulated the mRNA expression of whey acidic protein (WAP), lactoferrin and alpha-lactalbumin. In contrast, p-ethylphenol, daidzein and equol up-regulated beta-casein and/or WAP with alpha-lactalbumin. Furthermore, coumestrol and genistein down-regulated the expression of prolactin receptor and signal transducer and activator of transcription 5 (STAT5) accompanied by a decrease in STAT5 phosphorylation. Conclusion: Isoflavones and their metabolites influence the milk production ability of MECs through different interactions with prolactin/STAT5 signaling. Simultaneous intake of multiple isoflavones by consumption of legumes may induce promotive or adverse effects on lactating MECs.
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
This is the peer reviewed version of the following article:Molecular Nutrition & Food Research; 61(10) 1700156; Oct 2017, which has been published in final form at https://doi.org/10.1002/mnfr.201700156. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
PISSN
1613-4125
EISSN
1613-4133
NCID
AA11974242
関連情報
PMID
28605125
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1002/mnfr.201700156