Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis

Sugiura, Ryo; Ohnishi, Shunsuke; Ohara, Masatsugu; Ishikawa, Marin; Miyamoto, Shuichi; Onishi, Reizo; Yamamoto, Koji; Kawakubo, Kazumichi; Kuwatani, Masaki; Sakamoto, Naoya

Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis

Sugiura, Ryo; Ohnishi, Shunsuke; Ohara, Masatsugu et al.

American journal of translational research, 2018, 10(7), 2102-2114

Permalink : https://hdl.handle.net/2115/71629

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アクセス権	open access
URI	https://hdl.handle.net/2115/71629
タイトル	Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis
著者	Sugiura, Ryo ; Ohnishi, Shunsuke ; Ohara, Masatsugu ; Ishikawa, Marin ; Miyamoto, Shuichi ; Onishi, Reizo ; Yamamoto, Koji ; Kawakubo, Kazumichi ; Kuwatani, Masaki ; Sakamoto, Naoya
著者名 Sugiura, Ryo
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Ohara, Masatsugu
著者名 Ishikawa, Marin
著者名 Miyamoto, Shuichi
著者名 Onishi, Reizo
著者名 Yamamoto, Koji
e-Rad_Researcher 80633578 著者名 Kawakubo, Kazumichi
e-Rad_Researcher 50431375 著者名 Kuwatani, Masaki
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
キーワード	Mesenchymal stem cells
	sclerosing cholangitis
	amnion
	alpha-naphthylisothiocyanate
	regenerative medicine
発行日	2018-07-30
出版者	e-Century Publishing Corporation
収録物名	American journal of translational research
巻(号)	10(7)
ページ	2102-2114
抄録	Mesenchymal stem cells (MSCs) represent a valuable cell source in regenerative medicine, and large numbers of MSCs can be isolated from the amnion noninvasively. Sclerosing cholangitis is a chronic cholestatic disease and characterized by progressive biliary destruction leading to cirrhosis. Many factors are involved in the development of sclerosing cholangitis; however, effective medical therapy is not established. We investigated the effects of human amnion-derived MSCs (hAMSCs) and conditioned medium (CM) obtained from hAMSC cultures in rats with sclerosing cholangitis. Sclerosing cholangitis was induced via the intragastric administration of 100 mg/kg alpha-naphthylisothiocyanate (ANIT) twice weekly for 4 weeks. One million hAMSCs or 200 mu L of CM were intravenously administered on days 15 and 22. Rats were sacrificed on day 29 and evaluated via histological, immunohistochemical, and mRNA expression analyses. hAMSC transplantation and CM administration significantly improved the histological score. In addition, these two interventions significantly improved biliary hyperplasia, peribiliary fibrosis, and inflammation in Glisson's sheath. Accordingly, CK19, MMP-9, and TNF-alpha, and MCP-1 expression in the liver was also decreased by hAMSC and CM administration. In conclusion, hAMSC and CM administration ameliorated biliary hyperplasia, peribiliary fibrosis, and inflammation in a rat model of sclerosing cholangitis. hAMSCs and CM may represent new modalities for treating sclerosing cholangitis.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 4.0 International
PISSN	1943-8141
関連情報	PMID 30093947
関連情報 (isIdenticalTo)	URI https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079143/