open access

https://hdl.handle.net/2115/71638

An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein

英語

oxidation

2017-10-05

Wiley-Blackwell

Chembiochem

18(19)

1903-1909

Although widely occurring lipid oxidation, which is triggered by reactive oxygen species (ROS), produces a variety of oxidized lipids, practical methods to efficiently analyze oxidized lipids remain elusive. Herein, it is shown that the glycoblotting platform can be used to analyze oxidized lipids. Analysis is based on the principle that lipid aldehydes, one of the oxidized lipid species, can be captured selectively, enriched, and detected. Moreover, 3-methyl-1-p-tolyltriazene (MTT) methylates phosphoric and carboxylic acids, and this MTT-mediated methylation is, in combination with conventional tandem mass spectrometry (MS/MS) analysis, an effective method for the structural analysis of oxidized lipids. By using three classes of standards, liposomes, and a lipoprotein, it is demonstrated that glycoblotting represents a powerful approach for focused lipidomics, even in complex macromolecules.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Furukawa, T., Hinou, H., Takeda, S., Chiba, H., Nishimura, SI., Hui, SP., An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein. Chembiochem, 2017; 18(19):1903-1909., which has been published in final form at 10.1002/cbic.201700384. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Self-Archiving.

1439-4227

1439-7633