Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification

MAHMOUDIAN, Laili; MELIN, Jonas; MOHAMADI, Mohamad Reza et al.

Analytical Sciences, 2008, 24(3), 327-332

Permalink : https://hdl.handle.net/2115/71665

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/71665
タイトル
Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification
著者
MAHMOUDIAN, Laili ; MELIN, Jonas ; MOHAMADI, Mohamad Reza ; YAMADA, Keiko ; OHTA, Michio ; KAJI, Noritada ; TOKESHI, Manabu ; NILSSON, Mats ; BABA, Yoshinobu
著者名
MAHMOUDIAN, Laili
著者名
MELIN, Jonas
著者名
MOHAMADI, Mohamad Reza
著者名
YAMADA, Keiko
著者名
OHTA, Michio
著者名
KAJI, Noritada
e-Rad_Researcher
60311437
著者名
TOKESHI, Manabu
著者名
NILSSON, Mats
著者名
BABA, Yoshinobu
言語
英語
キーワード
Microchip Electrophoresis
発行日
2008-03-10
出版者
日本分析化学会
収録物名
Analytical Sciences
巻(号)
24(3)
ページ
327-332
抄録
We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37°C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
EISSN
1348-2246
NCID
AA10500785
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.24.327