Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence

KAMIDATE, Tamio; KIKUCHI, Nobuhito; ISHIDA, Akihiko et al.

Analytical Sciences, 2005, 21(6), 701-704

Permalink : https://hdl.handle.net/2115/71701

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アクセス権
open access
URI
https://hdl.handle.net/2115/71701
タイトル
Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence
著者
KAMIDATE, Tamio ; KIKUCHI, Nobuhito ; ISHIDA, Akihiko ; TANI, Hirofumi
著者名
KAMIDATE, Tamio
著者名
KIKUCHI, Nobuhito
著者名
ISHIDA, Akihiko
e-Rad_Researcher
10271644
著者名
TANI, Hirofumi
言語
英語
キーワード
Homogentisic acid γ-lactone (HAL)
chemiluminescence (CL)
determination of horseradish peroxidase (HRP)
発行日
2005-06
出版者
日本分析化学会
収録物名
Analytical Sciences
巻(号)
21(6)
ページ
701-704
抄録
Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
EISSN
1348-2246
NCID
AA10500785
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.21.701