Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components

KAMIDATE, Tamio; FUJITA, Taigo; TANI, Hirofumi et al.

Analytical Sciences, 1998, 14(6), 1115-1119

Permalink : https://hdl.handle.net/2115/71705

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論文情報

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アクセス権
open access
URI
https://hdl.handle.net/2115/71705
タイトル
Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components
著者
KAMIDATE, Tamio ; FUJITA, Taigo ; TANI, Hirofumi ; WATANABE, Hiroto
著者名
KAMIDATE, Tamio
著者名
FUJITA, Taigo
e-Rad_Researcher
10271644
著者名
TANI, Hirofumi
著者名
WATANABE, Hiroto
言語
英語
キーワード
Firefly bioluminescence
adenosine-5'-triphosphate
liposome
stearyltrimethylammonium chloride
stearylamine
membrane surface potential
発行日
1998-12
出版者
日本分析化学会
収録物名
Analytical Sciences
巻(号)
14(6)
ページ
1115-1119
抄録
The effect of cationic components in liposome on the bioluminescence (BL) intensity from the firefly luciferin-luciferase reaction with ATP was investigated by use of vesicles formed by the extrusion technique (VET). Stearyltrimethyl-ammonium chloride (STAC), stearylamine (SA) and tetracaine (TC) were used as cationic components. The VET containing STAC and SA enhanced the maximum BL intensity. In contrast, a lowering of the maximum BL intensity was observed in the presence of the VET containing TC. The sensitivity for ATP in the presence of the VET containing STAC was improved by a factor of 2 and 10 times compared to that in the presence of the VET containing SA and that in water alone, respectively. The differences in the BL enhancement between cationic components could be explained in terms of different membrane surface potentials of the VET containing these cationc components.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
EISSN
1348-2246
NCID
AA10500785
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.14.1115