Equilibrium Study on Interactions between Proteins and Bile-Salt Micelles by Micellar Electrokinetic Chromatography

SAITOH, Tohru; FUKUDA, Teruyuki; TANI, Hirofumi et al.

Analytical Sciences, 1996, 12(4), 569-573

Permalink : https://hdl.handle.net/2115/71708

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アクセス権
open access
URI
https://hdl.handle.net/2115/71708
タイトル
Equilibrium Study on Interactions between Proteins and Bile-Salt Micelles by Micellar Electrokinetic Chromatography
著者
SAITOH, Tohru ; FUKUDA, Teruyuki ; TANI, Hirofumi ; KAMIDATE, Tamio ; WATANABE, Hiroto
著者名
SAITOH, Tohru
著者名
FUKUDA, Teruyuki
e-Rad_Researcher
10271644
著者名
TANI, Hirofumi
著者名
KAMIDATE, Tamio
著者名
WATANABE, Hiroto
言語
英語
キーワード
Protein
bile-salt micelle
binding constant
micellar electrokinetic chromatography
発行日
1996-08-10
出版者
日本分析化学会
収録物名
Analytical Sciences
巻(号)
12(4)
ページ
569-573
抄録
Interactions between proteins and bile-salt micelles were evaluated on the basis of the binding constants, which were obtained from the migration of proteins in micellar electrokinetic chromatography. The binding constants, Kb=[Pb]/ ([Pw][B]), where [Pb] is the concentration of a protein binding with a bile-salt, [Pw] free protein concentration, and [B] the concentration of bile salt present in the micelles, were successfully determined from the slope of a linear curve of the capacity factor (k′) of the protein against [B]. The binding constants were almost identical to those obtained by a gel- filtration method. The value of Kb increased with increasing the hydrophobicity of the bile salt or that of the protein.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
EISSN
1348-2246
NCID
AA10500785
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.12.569