Polymer-Induced Phase Separation in Aqueous Micellar Solutions of Alkylglucosides for Protein Extraction

SAITOH, Tohru; TANI, Hirofumi; KAMIDATE, Tamio et al.

Analytical Sciences, 1994, 10(2), 299-303

Permalink : https://hdl.handle.net/2115/71709

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アクセス権
open access
URI
https://hdl.handle.net/2115/71709
タイトル
Polymer-Induced Phase Separation in Aqueous Micellar Solutions of Alkylglucosides for Protein Extraction
著者
SAITOH, Tohru ; TANI, Hirofumi ; KAMIDATE, Tamio ; KAMATAKI, Tetsuya ; WATANABE, Hiroto
著者名
SAITOH, Tohru
e-Rad_Researcher
10271644
著者名
TANI, Hirofumi
著者名
KAMIDATE, Tamio
著者名
KAMATAKI, Tetsuya
著者名
WATANABE, Hiroto
言語
英語
キーワード
phase separation
alkylglucoside
polymer
protein extraction
発行日
1994-04
出版者
日本分析化学会
収録物名
Analytical Sciences
巻(号)
10(2)
ページ
299-303
抄録
Aqueous micellar solutions of alkylglucosides were separated into two phases at 0°C upon addition of polyethylene glycol 6000 (PEG) or Dextran T-500 (Dextran). One was an aqueous phase in which hydrophilic proteins, cytochrome c and peroxidase (horseradish), were retained, and the other was a surfactant-rich phase into which hydrophobic membrane proteins, bacteriorhodopsin and cytochrome b5, were extracted. A combination of octyl-β-D-thioglucoside (OTG) (or nonyl-β-D-glucoside (NG)) with PEG (or Dextran) was the best choice for extraction of hydrophobic proteins. Extraction yields (50-90%) and concentration factors (7-30) of the hydrophobic proteins were dependent on the types of nonionic surfactants and water-soluble polymers. Solubilization and phase separation in processing cell membranes could be made in a single step at 0°C. Hence the present method would be useful for the purification of thermolabile proteins.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
PISSN
0910-6340
EISSN
1348-2246
NCID
AA10500785
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.2116/analsci.10.299