Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes

KAMIDATE, Tamio; MARUYA, Masumi; TANI, Hirofumi; ISHIDA, Akihiko

Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes

KAMIDATE, Tamio; MARUYA, Masumi; TANI, Hirofumi et al.

Analytical Sciences, 2009, 25(9), 1163-1166

Permalink : https://hdl.handle.net/2115/71739

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アクセス権	open access
URI	https://hdl.handle.net/2115/71739
タイトル	Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes
著者	KAMIDATE, Tamio ; MARUYA, Masumi ; TANI, Hirofumi ; ISHIDA, Akihiko
著者名 KAMIDATE, Tamio
著者名 MARUYA, Masumi
著者名 TANI, Hirofumi
著者名 ISHIDA, Akihiko
言語	英語
キーワード	horseradish peroxidase (HRP)
キーワード	chemiluminescence (CL)
発行日	2009-09-10
出版者	日本分析化学会
収録物名	Analytical Sciences
巻(号)	25(9)
ページ	1163-1166
抄録	4-Iodophenol was applied to an enhancer in the direct detection of horseradish peroxidase (HRP) encapsulated in liposomes by using luminol chemiluminescence (CL). Luminol, 4-iodophenol and hydrogen peroxide permeate into the inner phase of liposomes containing HRP, resulting in the progress of 4-iodophenol-enhanced luminol CL catalyzed by HRP in liposomes. The CL intensity observed in liposomes was a factor of 150 greater than that observed in a lipid-free bulk solution. The detection limit in the direct detection of HRP encapsulated in liposomes was sensitive by a factor of 30 compared with that in a lipid-free bulk solution. 4-Iodophenol effectively functioned as an enhancer in HRP-catalyzed luminol CL in liposomes.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0910-6340
EISSN	1348-2246
NCID	AA10500785
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2116/analsci.25.1163