open access

https://hdl.handle.net/2115/71744

Microfluidic Approaches for Protein Crystal Structure Analysis

英語

in-situ X-ray analysis

2016-01-10

日本分析化学会

Analytical Sciences

32(1)

3-9

This review summarizes two microfluidic-based protein crystallization methods, protein crystallization behavior in the microfluidic devices, and their applications for X-ray crystal structure analysis. Microfluidic devices provide many advantages for protein crystallography; they require small sample volumes, provide high-throughput screening, and allow control of the protein crystallization. A droplet-based protein crystallization method is a useful technique for high-throughput screening and the formation of a single crystal without any complicated device fabrication process. Well-based microfluidic platforms also enable effective protein crystallization. This review also summarizes the protein crystal growth behavior in microfluidic devices as, is known from viewpoints of theoretical and experimental approaches. Finally, we introduce applications of microfluidic devices for on-chip crystal structure analysis.

学術雑誌論文

Version of Record

0910-6340

1348-2246

AA10500785