open access

https://hdl.handle.net/2115/71760

Dynamics and function of ERM proteins during cytokinesis in human cells

英語

2017-10-26

FEBS Letters

591(20)

3296-3309

The molecular mechanism that governs cytoskeleton-membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane-associated domain in a cholesterol-dependent but largely Rho-independent manner. While ERMs are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar cortex. Notably, when anillin and supervillin are codepleted, ERMs increasingly accumulate at the cleavage furrow and substantially contribute to the furrow ingression. These results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Shota Hiruma, Tomoko Kamasaki, Kohei Otomo, Tomomi Nemoto and Ryota Uehara, Dynamics and function of ERM proteins during cytokinesis in human cells, FEBS Letters 591(20), 2017, doi: 10.1002/1873-3468.12844, which has been published in final form at http://dx.doi.org/10.1002/1873-3468.12844 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

0014-5793