An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)

Sato, Kousuke; Kawamoto, Kyoji; Shimamura, Shintaro; Ichikawa, Satoshi; Matsuda, Akira

An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)

Sato, Kousuke; Kawamoto, Kyoji; Shimamura, Shintaro et al.

Bioorganic & Medicinal Chemistry Letters, 2016, 26(22), 5395-5398

Permalink : https://hdl.handle.net/2115/71972

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アクセス権	open access
URI	https://hdl.handle.net/2115/71972
タイトル	An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)
著者	Sato, Kousuke ; Kawamoto, Kyoji ; Shimamura, Shintaro ; Ichikawa, Satoshi ; Matsuda, Akira
e-Rad_Researcher 70415686 著者名 Sato, Kousuke
著者名 Kawamoto, Kyoji
著者名 Shimamura, Shintaro
e-Rad_Researcher 60333621 著者名 Ichikawa, Satoshi
e-Rad_Researcher 90157313 著者名 Matsuda, Akira
言語	英語
キーワード	Epigenetic
	Nucleic acids
	5-Formyl-2 '-deoxycytidine
	Covalent bond
	DNA methyltransferase
発行日	2016-11-15
出版者	Elsevier
収録物名	Bioorganic & Medicinal Chemistry Letters
巻(号)	26(22)
ページ	5395-5398
抄録	5-Methylcytosine (mC) is known to induce epigenetic changes. Ten-eleven translocation (TET) enzymes produce the further oxidized 5-substituted cytosine derivatives, 5-formylcytosine (fC) and 5-carboxylcytosine (caC). However, their roles are unclear thus far. Here, we synthesized oligodeoxyribonucleotides (ODNs) containing 5-formyl-2'-deoxycytidine and examined their interactions with DNA cytosine-5 methyltransferase (DNMT). We found that the ODN sequence containing fCpG formed a covalent complex with both bacterial and mouse recombinant DNMTs in the absence of any cofactors. The covalent bonding with DNMT suggests that the fCpG sequence in DNA may play a role in epigenetic regulation. (C) 2016 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0960-894X
関連情報	PMID 27780634
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.bmcl.2016.10.040