Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus

Wu, Yu-Er; Enoki, Ryosuke; Oda, Yoshiaki; Huang, Zhi-Li; Honma, Ken-ichi; Honma, Sato

Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus

Wu, Yu-Er; Enoki, Ryosuke; Oda, Yoshiaki et al.

Proceedings of the National Academy of Sciences of the United States of America (PNAS), 2018, 115(40), E9469-E9478

Permalink : https://hdl.handle.net/2115/71975

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アクセス権	open access
URI	https://hdl.handle.net/2115/71975
タイトル	Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus
著者	Wu, Yu-Er ; Enoki, Ryosuke ; Oda, Yoshiaki ; Huang, Zhi-Li ; Honma, Ken-ichi ; Honma, Sato
著者名 Wu, Yu-Er
e-Rad_Researcher 00528341 著者名 Enoki, Ryosuke
著者名 Oda, Yoshiaki
著者名 Huang, Zhi-Li
著者名 Honma, Ken-ichi
著者名 Honma, Sato
言語	英語
キーワード	intracellular calcium
	fluorescence imaging
	neuronal network
	ultradian rhythm
	circadian rhythm
発行日	2018-10-02
出版者	National Academy of Sciences.
収録物名	Proceedings of the National Academy of Sciences of the United States of America (PNAS)
巻(号)	115(40)
ページ	E9469-E9478
抄録	The suprachiasmatic nucleus (SCN), the master circadian clock in mammals, sends major output signals to the subparaventricular zone (SPZ) and further to the paraventricular nucleus (PVN), the neural mechanism of which is largely unknown. In this study, the intracellular calcium levels were measured continuously in cultured hypothalamic slices containing the PVN, SPZ, and SCN. We detected ultradian calcium rhythms in both the SPZ-PVN and SCN regions with periods of 0.5-4.0 hours, the frequency of which depended on the local circadian rhythm in the SPZ-PVN region. The ultradian rhythms were synchronous in the entire SPZ-PVN region and a part of the SCN. Because the ultradian rhythms were not detected in the SCN-only slice, the origin of ultradian rhythm is the SPZ-PVN region. In association with an ultradian bout, a rapid increase of intracellular calcium in a millisecond order was detected, the frequency of which determined the amplitude of an ultradian bout. The synchronous ultradian rhythms were desynchronized and depressed by a sodium channel blocker tetrodotoxin, suggesting that a tetrodotoxin-sensitive network is involved in synchrony of the ultradian bouts. In contrast, the ultradian rhythm is abolished by glutamate receptor blockers, indicating the critical role of glutamatergic mechanism in ultradian rhythm generation, while a GABA(A) receptor blocker increased the frequency of ultradian rhythm and modified the circadian rhythm in the SCN. A GABAergic network may refine the circadian output signals. The present study provides a clue to unraveling the loci and network mechanisms of the ultradian rhythm.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0027-8424
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1073/pnas.1804300115