open access

https://hdl.handle.net/2115/72007

Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence

英語

in vitro growth

2017-11

Japanese Society of Animal Science

Animal Science Journal

88(11)

1686-1691

Bone morphogenetic protein-4 (BMP-4) inhibits luteinization of granulosa cells during in vitro growth (IVG) culture of bovine oocytes; however, oocytes derived from a 12-day IVG were less competent for development than in vivo-grown oocytes. We herein investigated whether an extended IVG culture with BMP-4 improves oocyte growth and development to blastocysts after in vitro fertilization. Oocyte-granulosa cell complexes (OGCs) were cultured for 14 or 16 days with BMP-4 (10 ng/mL), while a 12-day culture with BMP-4 served as the in vitro control. OGC viability was maintained for the 16-day culture with BMP-4 (83.2%), but was significantly lower without BMP-4 (58.9%) than the control (83.0%). Prolong-cultured oocytes at 16 days had statistically greater diameter (114.6 μm) than the control (111.7 μm). IVG oocytes with BMP-4 for the 16-day culture had a similar nuclear maturation rate to the control (approximately 67%); however, blastocyst rates in BMP-4 treated oocytes of 14- (1.8%) and 16-day (0%) IVG were statistically lower than that of 12-day IVG (9.0%). In conclusion, BMP-4 maintained OGC viability and promoted oocyte growth in a prolonged culture, but impaired the developmental competence of oocytes. Prolonged culture may not be an appropriate strategy for enhancing the developmental competence of IVG oocytes.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Yang, Y., Kanno, C., Sakaguchi, K., Yanagawa, Y., Katagiri, S., and Nagano, M. (2017) Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence. Anim Sci J, 88: 1686–1691. doi: 10.1111/asj.12841., which has been published in final form at https://doi.org/10.1111/asj.12841. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

1344-3941