Superior Microvascular Perfusion of Infused Liposome-Encapsulated Hemoglobin Prior to Reductions in Infarctions after Transient Focal Cerebral Ischemia

Shimbo, Daisuke; Abumiya, Takeo; Kurisu, Kota; Osanai, Toshiya; Shichinohe, Hideo; Nakayama, Naoki; Kazumata, Ken; Nakamura, Hideki; Shimuzu, Hiroshi; Houkin, Kiyohiro

Superior Microvascular Perfusion of Infused Liposome-Encapsulated Hemoglobin Prior to Reductions in Infarctions after Transient Focal Cerebral Ischemia

Shimbo, Daisuke; Abumiya, Takeo; Kurisu, Kota et al.

Journal of stroke & cerebrovascular diseases, 2017, 26(12), 2994-3003

Permalink : https://hdl.handle.net/2115/72077

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アクセス権	open access
URI	https://hdl.handle.net/2115/72077
タイトル	Superior Microvascular Perfusion of Infused Liposome-Encapsulated Hemoglobin Prior to Reductions in Infarctions after Transient Focal Cerebral Ischemia
著者	Shimbo, Daisuke ; Abumiya, Takeo ; Kurisu, Kota ; Osanai, Toshiya ; Shichinohe, Hideo ; Nakayama, Naoki ; Kazumata, Ken ; Nakamura, Hideki ; Shimuzu, Hiroshi ; Houkin, Kiyohiro
著者名 Shimbo, Daisuke
e-Rad_Researcher 80270726 著者名 Abumiya, Takeo
著者名 Kurisu, Kota
e-Rad_Researcher 90622788 著者名 Osanai, Toshiya
e-Rad_Researcher 80374479 著者名 Shichinohe, Hideo
e-Rad_Researcher 40421961 著者名 Nakayama, Naoki
著者名 Kazumata, Ken
e-Rad_Researcher 60435956 著者名 Nakamura, Hideki
著者名 Shimuzu, Hiroshi
e-Rad_Researcher 90229146 著者名 Houkin, Kiyohiro
言語	英語
キーワード	Infarction
	transient focal cerebral ischemia
	microvessels
	neuroprotection
発行日	2017-12
出版者	Elsevier
収録物名	Journal of stroke & cerebrovascular diseases
巻(号)	26(12)
ページ	2994-3003
抄録	Background: The development of cerebral infarction after transient ischemia is attributed to postischemic delayed hypoperfusion in the microvascular region. In the present study, we assessed the microvascular perfusion capacity of infused liposome-encapsulated hemoglobin (LEH) in a therapeutic approach for transient middle cerebral artery occlusion (tMCAO). Methods: Two-hour middle cerebral artery occlusion rats were immediately subjected to intra-arterial infusion of LEH (LEH group) or saline (vehicle group) or no treatment (control group), and then to recanalization. Neurological findings, infarct and edema progression, microvascular endothelial dysfunction, and inflammatory reactions were compared between the 3 groups after 24 hours of reperfusion. Microvascular perfusion in the early phase of reperfusion was evaluated by hemoglobin immunohistochemistry and transmission electron microscopy. Results: The LEH group achieved significantly better results in all items evaluated than the other groups. Hemoglobin immunohistochemistry revealed that the number of hemoglobin-positive microvessels was significantly greater in the LEH group than in the other groups (P < .01), with microvascular perfusion being more likely in narrow microvessels (≤ 5 µm in diameter). An electron microscopic examination revealed that microvessels in the control group were compressed and narrowed by swollen astrocyte end-feet, whereas those in the LEH group had a less deformed appearance and contained LEH particles and erythrocytes. Conclusion: The results of the present study demonstrated that the infusion of LEH reduced infarctions after tMCAO with more hemoglobin-positive and less deformed microvessels at the early phase of reperfusion, suggesting that the superiority of the microvascular perfusion of LEH mediates its neuroprotective effects.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1052-3057
関連情報	PMID 28843805
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jstrokecerebrovasdis.2017.07.026