Evaluation of the chemiluminescent enzyme immunoassay system for the measurement of testosterone in the serum and whole blood of stallions

Toishi, Yuko; Tsunoda, Nobuo; Nagata, Shun-ichi; Kirisawa, Rikio; Nagaoka, Kentaro; Watanabe, Gen; Yanagawa, Yojiro; Katagiri, Seiji; Taya, Kazuyoshi

Evaluation of the chemiluminescent enzyme immunoassay system for the measurement of testosterone in the serum and whole blood of stallions

Toishi, Yuko; Tsunoda, Nobuo; Nagata, Shun-ichi et al.

Journal of reproduction and development, 2018, 64(1), 41-47

Permalink : https://hdl.handle.net/2115/72140

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アクセス権	open access
URI	https://hdl.handle.net/2115/72140
タイトル	Evaluation of the chemiluminescent enzyme immunoassay system for the measurement of testosterone in the serum and whole blood of stallions
著者	Toishi, Yuko ; Tsunoda, Nobuo ; Nagata, Shun-ichi ; Kirisawa, Rikio ; Nagaoka, Kentaro ; Watanabe, Gen ; Yanagawa, Yojiro ; Katagiri, Seiji ; Taya, Kazuyoshi
著者名 Toishi, Yuko
著者名 Tsunoda, Nobuo
著者名 Nagata, Shun-ichi
著者名 Kirisawa, Rikio
e-Rad_Researcher 60376564 著者名 Nagaoka, Kentaro
e-Rad_Researcher 90158626 著者名 Watanabe, Gen
著者名 Yanagawa, Yojiro
e-Rad_Researcher 00292061 著者名 Katagiri, Seiji
著者名 Taya, Kazuyoshi
言語	英語
キーワード	Chemiluminescent enzyme immunoassay
	Cryptorchidism
	Reproductive abnormalities
	Stallions
	Testosterone
発行日	2018-02
出版者	Society for Reproduction and Development（SRD）
収録物名	Journal of reproduction and development
巻(号)	64(1)
ページ	41-47
抄録	Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions. The serum T concentrations measured by the two methods were highly correlated (r = 0.9865, n = 56). Second, to validate the use of whole blood as assay samples, T concentrations in whole blood and in the serum were measured by the CLEIA system. T concentrations in both samples were highly correlated (r = 0.9665, n = 64). Finally, to evaluate the practical value of the CLEIA system in clinical settings, T concentrations were measured in three stallions with reproductive abnormalities after the administration of human chorionic gonadotropin (hCG). Two stallions with small or absent testes in the scrotum showed an increase in T production in response to hCG administration and one stallion with seminoma did not. In conclusion, the CLEIA system was found to be a rapid and reliable tool for measuring T concentrations in stallions and may improve reproductive management in clinical settings and in breeding studs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-8818
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2017-099