Ovarian mast cells migrate toward ovary- fimbria connection in neonatal MRL/MpJ mice

Nakamura, Teppei; Chihara, Masataka; Ichii, Osamu; Otsuka-Kanazawa, Saori; Nagasaki, Ken-ichi; Elewa, Yaser Hosny Ali; Tatsumi, Osamu; Kon, Yasuhiro

Ovarian mast cells migrate toward ovary- fimbria connection in neonatal MRL/MpJ mice

Nakamura, Teppei; Chihara, Masataka; Ichii, Osamu et al.

PLoS ONE, 2018, 13(4), e0196364

Permalink : https://hdl.handle.net/2115/72160

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アクセス権	open access
URI	https://hdl.handle.net/2115/72160
タイトル	Ovarian mast cells migrate toward ovary- fimbria connection in neonatal MRL/MpJ mice
著者	Nakamura, Teppei ; Chihara, Masataka ; Ichii, Osamu ; Otsuka-Kanazawa, Saori ; Nagasaki, Ken-ichi ; Elewa, Yaser Hosny Ali ; Tatsumi, Osamu ; Kon, Yasuhiro
著者名 Nakamura, Teppei
著者名 Chihara, Masataka
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
e-Rad_Researcher 10566152 著者名 Otsuka-Kanazawa, Saori
著者名 Nagasaki, Ken-ichi
ORCID 0000-0002-7347-4587 著者名 Elewa, Yaser Hosny Ali
著者名 Tatsumi, Osamu
e-Rad_Researcher 10178402 著者名 Kon, Yasuhiro
言語	英語
発行日	2018-04-23
出版者	PLOS
収録物名	PLoS ONE
巻(号)	13(4)
ページ	e0196364
抄録	MRL/MpJ mice have abundant ovarian mast cells (MCs) as compared with other strains at postnatal day 0 (P0); however, they sharply decrease after birth. These ovarian MCs, particularly beneath the ovarian surface epithelium (SE), which express mucosal MC (MMC) marker, might participate in early follicular development. This study investigated the changes in spatiotemporal distribution of MCs in the perinatal MRL/MpJ mouse ovaries. At P0 to P7, the MCs were densely localized to the ovary, especially their caudomedial region around the ovary-fimbria connection. The neonatal ovarian MCs showed intermediate characteristics of MMC and connective tissue MC (CTMC), and the latter phenotype became evident with aging. However, the expression ratio of the MMC to CTMC marker increased from P0 to P4 in the MRL/MpJ mouse ovary. Similarly, the ratio of MCs facing SE to total MC number increased with aging, although the number of ovarian MCs decreased, indicating the relative increase in MMC phenotypes in the early neonatal ovary. Neither proliferating nor apoptotic MCs were found in the MRL/MpJ mouse ovaries. The parenchymal cells surrounding MCs at ovary-fimbria connection showed similar molecular expression patterns (E-cadherin(+)/Foxl2(-)/Gata4(+)) as that of the ovarian surface epithelial cells. At P2, around the ovary-fimbria connection, c-kit - immature oocytes formed clusters called nests, and some MCs localized adjacent to c-kit oocytes within the nests. These results indicated that in postnatal MRL/MpJ mice, ovarian MCs changed their distribution by migrating toward the parenchymal cells composing ovary-fimbria connection, which possessed similar characteristics to the ovarian surface epithelium. Thus, we elucidated the spatiotemporal alterations of the ovarian MCs in MRL/MpJ mice, and suggested their importance during the early follicular development by migrating toward the ovary-fimbria connection. MRL/MpJ mice would be useful to elucidate the relationship between neonatal immunity and reproductive systems.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1932-6203
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0196364