Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice

Masuki, H.; Li, Minqi; Hasegawa, T.; Suzuki, R.; Guo, Y.; Liu, Z.; Oda, K.; Yamamoto, T.; Kawanami, M.; Amizuka, N.

Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice

Masuki, H.; Li, Minqi; Hasegawa, T. et al.

Biomedical Research, 2010, 31(5), 307-318

Permalink : https://hdl.handle.net/2115/72271

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アクセス権	open access
URI	https://hdl.handle.net/2115/72271
タイトル	Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice
著者	Masuki, H. ; Li, Minqi ; Hasegawa, T. ; Suzuki, R. ; Guo, Y. ; Liu, Z. ; Oda, K. ; Yamamoto, T. ; Kawanami, M. ; Amizuka, N.
著者名 Masuki, H.
e-Rad_Researcher 60447612 著者名 Li, Minqi
e-Rad_Researcher 50739349 著者名 Hasegawa, T.
e-Rad_Researcher 90333723 著者名 Suzuki, R.
著者名 Guo, Y.
著者名 Liu, Z.
e-Rad_Researcher 10122681 著者名 Oda, K.
e-Rad_Researcher 80200822 著者名 Yamamoto, T.
e-Rad_Researcher 10133761 著者名 Kawanami, M.
e-Rad_Researcher 30242431 著者名 Amizuka, N.
言語	英語
発行日	2010-11-10
出版者	Biomedical Research Press
収録物名	Biomedical Research
巻(号)	31(5)
ページ	307-318
抄録	In order to define the osteocytic function in accelerated bone remodeling, we examined the distribution of the osteocytic lacunar-canalicular system (OLCS) and osteocyte-secreting molecules-dentin matrix protein (DMP) 1 and sclerostin-in the epiphyses and cortical bones of osteoprotegerin deficient (OPG-/-) mice. Silver impregnation visualized a well-arranged OLCS in the wild-type epiphyses and cortical bone, whereas OPG-/- mice had an irregular OLCS in the epiphyses, but well-arranged canaliculi in the cortical bone. DMP1-positive osteocytes were evenly distributed throughout the wild-type epiphyses and cortical bone, as well as the OPG-/- cortical bone. However, OPG-/- epiphyses revealed weak DMP1-immunoreactivity. Thus, osteocytes appear to synthesize more DMP1 as the OLCS becomes regular. In contrast, sclerostin-immunoreactivity was significantly diminished in the OPG-/- epiphyses and cortical bone. In OPG-/- epiphyses and cortical bone, triple staining demonstrated few sclerostin-positive osteocytes in the periphery of a thick cell layer of alkaline phosphatase-positive osteoblasts and many tartrate resistant acid phosphatasepositive osteoclasts. Summarizing, the regular distribution of OLCS may affect DMP1 synthesis, while the cellular activities of osteoclasts and osteoblasts rather than the regularity of OLCS may ultimately influence sclerostin synthesis.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0388-6107
EISSN	1880-313X
NCID	AA00110128
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2220/biomedres.31.307