Sclerostin is differently immunolocalized in metaphyseal trabecules and cortical bones of mouse tibiae

Hasegawa, T.; Amizuka, N.; Yamada, T.; Liu, Z.; Miyamoto, Y.; Yamamoto, T.; Sasaki, M.; Hongo, H.; Suzuki, R.; Freitas, PHL.; Yamamoto, T.; Oda, K.; Li, M.

Sclerostin is differently immunolocalized in metaphyseal trabecules and cortical bones of mouse tibiae

Hasegawa, T.; Amizuka, N.; Yamada, T. et al.

Biomedical Research, 2013, 34(3), 153-159

Permalink : https://hdl.handle.net/2115/72293

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アクセス権	open access
URI	https://hdl.handle.net/2115/72293
タイトル	Sclerostin is differently immunolocalized in metaphyseal trabecules and cortical bones of mouse tibiae
著者	Hasegawa, T. ; Amizuka, N. ; Yamada, T. ; Liu, Z. ; Miyamoto, Y. ; Yamamoto, T. ; Sasaki, M. ; Hongo, H. ; Suzuki, R. ; Freitas, PHL. ; Yamamoto, T. ; Oda, K. ; Li, M.
e-Rad_Researcher 50739349 著者名 Hasegawa, T.
e-Rad_Researcher 30242431 著者名 Amizuka, N.
e-Rad_Researcher 80580943 著者名 Yamada, T.
著者名 Liu, Z.
著者名 Miyamoto, Y.
著者名 Yamamoto, T.
著者名 Sasaki, M.
著者名 Hongo, H.
e-Rad_Researcher 90333723 著者名 Suzuki, R.
著者名 Freitas, PHL.
e-Rad_Researcher 80200822 著者名 Yamamoto, T.
e-Rad_Researcher 10122681 著者名 Oda, K.
e-Rad_Researcher 60447612 著者名 Li, M.
言語	英語
発行日	2013-06-19
出版者	Biomedical Research Press
収録物名	Biomedical Research
巻(号)	34(3)
ページ	153-159
抄録	Sclerostin, an osteocyte-derived molecule, has been reported to serve as a negative regulator of osteoblastic activity as well as bone remodeling. However, there is no report that verified the regional difference for sclerostin synthesis, and in this study we have investigated immunolocalization of sclerostin by comparing dentin matrix protein (DMP) 1, an osteocyte-derived factor broadly expressed in tibial metaphyses and cortical bone. In metaphyseal primary trabecules, a site of bone modeling, strong DMP1-reactivity was observed in osteocytic lacunar-canalicular system (OLCS), while faint staining for sclerostin was visible only in a few osteocytes. In secondary trabecules, in which bone remodeling begins, some osteocytes showed intense sclerostin-immunopositivity, though there were many DMP1-positive osteocytes. In cortical bone, there were more osteocytes reactive for sclerostin, when compared with those in the secondary trabecules. Silver impregnation verified that immature, primary trabecules contained randomly-oriented OLCS, while mature, cortical bone showed geometrically well-arrangement of OLCS. Taken together, though DMP1 is broadly synthesized in bone, sclerostin appears to be abundantly synthesized in regular OLCS of cortical bone, but less produced in irregular OLCS as seen in primary trabecules, indicating the regional difference for sclerostin synthesis.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	0388-6107
EISSN	1880-313X
NCID	AA00110128
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.2220/biomedres.34.153