Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility

Kanaoka, Yoshitaka; Kuniyoshi, Daichi; Inada, Eri; Koide, Yohei; Okamoto, Yoshihiro; Yasui, Hideshi; Kishima, Yuji

Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility

Kanaoka, Yoshitaka; Kuniyoshi, Daichi; Inada, Eri et al.

Plant methods, 2018, 14, 102

Permalink : https://hdl.handle.net/2115/72300

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アクセス権	open access
URI	https://hdl.handle.net/2115/72300
タイトル	Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
著者	Kanaoka, Yoshitaka ; Kuniyoshi, Daichi ; Inada, Eri ; Koide, Yohei ; Okamoto, Yoshihiro ; Yasui, Hideshi ; Kishima, Yuji
著者名 Kanaoka, Yoshitaka
著者名 Kuniyoshi, Daichi
著者名 Inada, Eri
著者名 Koide, Yohei
著者名 Okamoto, Yoshihiro
著者名 Yasui, Hideshi
e-Rad_Researcher 60192556 著者名 Kishima, Yuji
言語	英語
キーワード	Anther culture
	Callus
	Hybrid sterility
	Mapping
	Microspore
	Oryza sativa
	O. glaberrima
	Rice
発行日	2018-11-17
出版者	BioMed Central
収録物名	Plant methods
巻(号)	14
ページ	102
抄録	BackgroundTo investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood.ResultsMicrospores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S-1 and S-19 loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD.ConclusionsWe demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
権利情報	© The Author(s) 2018
PISSN	1746-4811
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1186/s13007-018-0370-z