Production of recombinant salmon insulin-like growth factor binding protein-1 subtypes

Tanaka, Hanae; Oishi, Gakuto; Nakano, Yusuke; Mizuta, Hiroko; Nagano, Yuta; Hiramatsu, Naoshi; Ando, Hironori; Shimizu, Munetaka

Production of recombinant salmon insulin-like growth factor binding protein-1 subtypes

Tanaka, Hanae; Oishi, Gakuto; Nakano, Yusuke et al.

General and Comparative Endocrinology, 2018, 257, 184-191

Permalink : https://hdl.handle.net/2115/72472

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アクセス権	open access
URI	https://hdl.handle.net/2115/72472
タイトル	Production of recombinant salmon insulin-like growth factor binding protein-1 subtypes
著者	Tanaka, Hanae ; Oishi, Gakuto ; Nakano, Yusuke ; Mizuta, Hiroko ; Nagano, Yuta ; Hiramatsu, Naoshi ; Ando, Hironori ; Shimizu, Munetaka
著者名 Tanaka, Hanae
著者名 Oishi, Gakuto
著者名 Nakano, Yusuke
著者名 Mizuta, Hiroko
著者名 Nagano, Yuta
e-Rad_Researcher 10443920 著者名 Hiramatsu, Naoshi
著者名 Ando, Hironori
e-Rad_Researcher 90431337 著者名 Shimizu, Munetaka
言語	英語
キーワード	insulin-like growth factor binding protein-1
	recombinant protein
	salmon
	pituitary cell culture
	growth hormone release
発行日	2018-02-01
収録物名	General and Comparative Endocrinology
巻(号)	257
ページ	184-191
抄録	Insulin-like growth factor (IGF)-I is a growth promoting hormone that exerts its actions through endocrine, paracrine and autocrine modes. Local IGF-I is essential for normal growth, whereas circulating IGF-I plays a crucial role in regulating the production and secretion of growth hormone (GH) by the pituitary gland. These actions of IGF-I are modulated by six insulin-like growth factor binding proteins (IGFBPs). In teleosts, two subtypes of each IGFBP are present due to an extra round of whole-genome duplication. IGFBP-1 is generally inhibitory to IGF-I action under catabolic conditions such as fasting and stress. In salmon, IGFBP-1a and -1b are two of three major circulating IGFBPs and assumed to affect growth through modulating IGF-I action. However, exact functions of salmon IGFBP-1 subtypes on growth regulation are not known due to the lack of purified or recombinant protein. We expressed recombinant salmon (rs) IGFBP-1a and -1b with a fusion protein (thioredoxin, Trx) and a His-tag using the pET-32a(+) vector expression system in Escherichia coli. Trx.His.rsIGFBP-1s were isolated by Ni-affinity chromatography, enzymatically cleaved by enterokinase to remove the fusion partners and further purified by reversed-phase HPLC. We next examined effects of rsIGFBP-1a and -1b in combination with human IGF-I on GH release from cultured masu salmon (Oncorhynchus masou) pituitary cells. Unexpectedly, IGF-I increased GH release and an addition of rsIGFBP-1a, but not rsIGFBP-1b, restored GH levels. The results suggest that IGFBP-1a can inhibit IGF-I action on the pituitary in masu salmon. Availability of recombinant salmon IGFBP-1s should facilitate further functional analyses and assay development.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0016-6480
関連情報	PMID 28666856
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.ygcen.2017.06.015