Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells

Yamakawa, Kazuya; Matsuo, Junji; Okubo, Torahiko; Nakamura, Shinji; Yamaguchi, Hiroyuki

Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells

Yamakawa, Kazuya; Matsuo, Junji; Okubo, Torahiko et al.

Journal of infection and chemotherapy, 2018, 24(2), 130-137

Permalink : https://hdl.handle.net/2115/72706

このアイテムのアクセス数:872件（2026-08-15 13:57 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
J Infect Chemother_24(2)_130-137	pdf	690 KB	907

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/72706
タイトル	Impact of capsaicin, an active component of chili pepper, on pathogenic chlamydial growth (Chlamydia trachomatis and Chlamydia pneumoniae) in immortal human epithelial HeLa cells
著者	Yamakawa, Kazuya ; Matsuo, Junji ; Okubo, Torahiko ; Nakamura, Shinji ; Yamaguchi, Hiroyuki
著者名 Yamakawa, Kazuya
e-Rad_Researcher 50359486 著者名 Matsuo, Junji
著者名 Okubo, Torahiko
e-Rad_Researcher 40207882 著者名 Nakamura, Shinji
e-Rad_Researcher 40221650 著者名 Yamaguchi, Hiroyuki
言語	英語
キーワード	Chlamydia trachomatis
	Capsaicin
	HeLa cells
	TRPV1
	PPAR gamma
	LXR alpha
発行日	2018-02
出版者	Elsevier
収録物名	Journal of infection and chemotherapy
巻(号)	24(2)
ページ	130-137
抄録	Chlamydia trachomatis is the leading cause of sexually transmitted infections worldwide. Capsaicin, a component of chili pepper, which can stimulate actin remodeling via capsaicin receptor TRPV1 (transient receptor potential vanilloid 1) and anti-inflammatory effects via PPAR gamma (peroxisome proliferator-activated receptor-gamma) and LXR alpha (liver X receptor alpha), is a potential candidate to control chlamydial growth in host cells. We examined whether capsaicin could inhibit C. trachomatis growth in immortal human epithelial HeLa cells. Inclusion forming unit and quantitative PCR assays showed that capsaicin significantly inhibited bacterial growth in cells in a dose-dependent manner, even in the presence of cycloheximide, a eukaryotic protein synthesis inhibitor. Confocal microscopic and transmission electron microscopic observations revealed an obvious decrease in bacterial numbers to inclusions bodies formed in the cells. Although capsaicin can stimulate the apoptosis of cells, no increase in cleaved PARP (poly (ADP-ribose) polymerase), an apoptotic indicator, was observed at a working concentration. All of the drugs tested (capsazepine, a TRPV1 antagonist; 5CPPSS-50, an LXR alpha inhibitor; and T0070907, a PPAR gamma inhibitor) had no effect on chlamydial inhibition in the presence of capsaicin. In addition, we also confirmed that capsaicin inhibited Chlamydia pneumoniae growth, indicating a phenomena not specific to C. trachomatis. Thus, we conclude that capsaicin can block chlamydial growth without the requirement of host cell protein synthesis, but by another, yet to be defined, mechanism.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1341-321X
EISSN	1437-7780
関連情報	PMID 29132924
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.jiac.2017.10.007