The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection

Sasaki, Michihito; Anindita, Paulina D.; Ito, Naoto; Sugiyama, Makoto; Carr, Michael; Fukuhara, Hideo; Ose, Toyoyuki; Maenaka, Katsumi; Takada, Ayato; Hall, William W.; Orba, Yasuko; Sawa, Hirofumi

The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection

Sasaki, Michihito; Anindita, Paulina D.; Ito, Naoto et al.

The Journal of infectious diseases, 2018, 217(11), 1740-1749

Permalink : https://hdl.handle.net/2115/72895

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アクセス権	open access
URI	https://hdl.handle.net/2115/72895
タイトル	The Role of Heparan Sulfate Proteoglycans as an Attachment Factor for Rabies Virus Entry and Infection
著者	Sasaki, Michihito ; Anindita, Paulina D. ; Ito, Naoto ; Sugiyama, Makoto ; Carr, Michael ; Fukuhara, Hideo ; Ose, Toyoyuki ; Maenaka, Katsumi ; Takada, Ayato ; Hall, William W. ; Orba, Yasuko ; Sawa, Hirofumi
e-Rad_Researcher 70609403 著者名 Sasaki, Michihito
著者名 Anindita, Paulina D.
著者名 Ito, Naoto
著者名 Sugiyama, Makoto
著者名 Carr, Michael
著者名 Fukuhara, Hideo
e-Rad_Researcher 80380525 著者名 Ose, Toyoyuki
e-Rad_Researcher 10322752 著者名 Maenaka, Katsumi
e-Rad_Researcher 10292062 著者名 Takada, Ayato
著者名 Hall, William W.
e-Rad_Researcher 60507169 著者名 Orba, Yasuko
e-Rad_Researcher 30292006 著者名 Sawa, Hirofumi
言語	英語
キーワード	rabies virus
	heparan sulfate
	viral attachment
	viral glycoprotein
	host-virus interaction
発行日	2018-02-26
出版者	Oxford University Press
収録物名	The Journal of infectious diseases
巻(号)	217(11)
ページ	1740-1749
抄録	Rabies virus (RABV) is the causative agent of fatal neurological disease. Cellular attachment is the initial and essential step for viral infections. Although extensive studies have demonstrated that RABV uses various target cell molecules to mediate infection, no specific molecule has been identified as an attachment factor for RABV infection. Here we demonstrate that cellular heparan sulfate (HS) supports RABV adhesion and subsequent entry into target cells. Enzymatic removal of HS reduced cellular susceptibility to RABV infection, and heparin, a highly sulfated form of HS, blocked viral adhesion and infection. The direct binding between RABV glycoprotein and heparin was demonstrated, and this interaction was shown to require HS N- and 6-O-sulfation. We also revealed that basic amino acids in the ectodomain of RABV glycoprotein serve as major determinants for the RABV-HS interaction. Collectively, our study highlights a previously undescribed role of HS as an attachment factor for RABV infection.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The version of record [Volume 217, Issue 11, 5 May 2018] is available online at: https://doi.org/10.1093/infdis/jiy081.
PISSN	1537-6613
関連情報	PMID 29529215
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/infdis/jiy081