An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair : A preclinical proof-of-concept study

Tsujimoto, Takeru; Sudo, Hideki; Todoh, Masahiro; Yamada, Katsuhisa; Iwasaki, Koji; Ohnishi, Takashi; Hirohama, Naoki; Nonoyama, Takayuki; Ukeba, Daisuke; Ura, Katsuro; Ito, Yoichi M.; Iwasaki, Norimasa

An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair : A preclinical proof-of-concept study

Tsujimoto, Takeru; Sudo, Hideki; Todoh, Masahiro et al.

EBioMedicine, 2018, 37, 521-534

Permalink : https://hdl.handle.net/2115/72940

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/72940
タイトル	An acellular bioresorbable ultra-purified alginate gel promotes intervertebral disc repair : A preclinical proof-of-concept study
著者	Tsujimoto, Takeru ; Sudo, Hideki ; Todoh, Masahiro ; Yamada, Katsuhisa ; Iwasaki, Koji ; Ohnishi, Takashi ; Hirohama, Naoki ; Nonoyama, Takayuki ; Ukeba, Daisuke ; Ura, Katsuro ; Ito, Yoichi M. ; Iwasaki, Norimasa
著者名 Tsujimoto, Takeru
e-Rad_Researcher 30374367 著者名 Sudo, Hideki
e-Rad_Researcher 10314402 著者名 Todoh, Masahiro
著者名 Yamada, Katsuhisa
著者名 Iwasaki, Koji
著者名 Ohnishi, Takashi
著者名 Hirohama, Naoki
著者名 Nonoyama, Takayuki
著者名 Ukeba, Daisuke
著者名 Ura, Katsuro
e-Rad_Researcher 10334236 著者名 Ito, Yoichi M.
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
言語	英語
キーワード	intervertebral disc
	Biomaterials
	Biomechanics
	Tissue engineering
発行日	2018-11
出版者	Elsevier
収録物名	EBioMedicine
巻(号)	37
ページ	521-534
抄録	Background: The current surgical procedure of choice for lumbar intervertebral disc (IVD) herniation is discectomy. However, defects within IVD produced upon discectomy may impair tissue healing and predispose patients to subsequent IVD degeneration. This study aimed to investigate whether the use of an acellular bioresorbable ultra-purified alginate (UPAL) gel implantation system is safe and effective as a reparative therapeutic strategy after lumbar discectomy. Methods: Human IVD cells were cultured in a three-dimensional system in UPAL gel. In addition, lumbar spines of sheep were used for mechanical analysis. Finally, the gel was implanted into IVD after discectomy in rabbits and sheep in vivo. Findings: The UPAL gel was biocompatible with human IVD cells and promoted extracellular matrix production after discectomy, demonstrating sufficient biomechanical characteristics without material protrusion. Interpretation: The present results indicate the safety and efficacy of UPAL gels in a large animal model and suggest that these gels represent a novel therapeutic strategy after discectomy in cases of lumbar IVD herniation. Fund: Grant-in-Aid for the Ministry of Education, Culture, Sports, Science, and Technology of Japan, Japan Agency for Medical Research and Development, and the Mochida Pharmaceutical Co., Ltd.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	2352-3964
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.ebiom.2018.10.055