Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes

Sakaguchi, Kenichiro; Tanida, Takashi; Abdel-Ghani, Mohammed A.; Kanno, Chihiro; Yanagawa, Yojiro; Katagiri, Seiji; Nagano, Masashi

Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes

Sakaguchi, Kenichiro; Tanida, Takashi; Abdel-Ghani, Mohammed A. et al.

Journal of reproduction and development, 2018, 64(6), 503-510

Permalink : https://hdl.handle.net/2115/73353

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アクセス権	open access
URI	https://hdl.handle.net/2115/73353
タイトル	Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes
著者	Sakaguchi, Kenichiro ; Tanida, Takashi ; Abdel-Ghani, Mohammed A. ; Kanno, Chihiro ; Yanagawa, Yojiro ; Katagiri, Seiji ; Nagano, Masashi
著者名 Sakaguchi, Kenichiro
著者名 Tanida, Takashi
著者名 Abdel-Ghani, Mohammed A.
著者名 Kanno, Chihiro
著者名 Yanagawa, Yojiro
e-Rad_Researcher 00292061 著者名 Katagiri, Seiji
e-Rad_Researcher 70312402 著者名 Nagano, Masashi
言語	英語
キーワード	Antral follicle count
	Bovine oocyte
	In vitro growth
	Steroidogenesis
発行日	2018-12
出版者	Society for Reproduction and Development（SRD）
収録物名	Journal of reproduction and development
巻(号)	64(6)
ページ	503-510
抄録	The antral follicle count (AFC) is used as an indicator of cow fertility. We herein investigated the relationship between AFC and the steroidogenesis of granulosa cells and confirmed the developmental competence of oocytes derived from early antral follicles (0.5-1.0 mm) using in vitro growth culture. Slaughterhouse -derived ovaries were divided into high (>= 25) and low (< 25) AFC groups based on AFC (>= 2.0 mm). Oocyte-cumulus-granulosa complexes (OCGC5) collected from early antral follicles were cultured for 12 days. The total number, viability, and diameter of granulosa cells and estradiol-17 beta and progesterone production during the culture were evaluated. Surviving oocytes on day 12 were subjected to in vitro maturation, and their volume and nuclear status were evaluated. Some oocytes were subjected to the evaluation of developmental competence to blastocysts. Although the total number and viability of granulosa cells did not differ between the groups, granulosa cell diameters were smaller in the high AFC group than in the low AFC group. The estradiol-17 beta and progesterone ratio on day 8 was higher in the high AFC group than in the low AFC group. Oocyte volumes and nuclear maturation rates were greater in the high AFC group than in the low AFC group. The development rate to blastocysts was 9.1% in the high AFC group, while no oocytes developed to blastocysts in the low AFC group. Therefore, estradiol-17 beta production by granulosa cells appears to be greater in high AFC cattle than in low AFC cattle, thereby promoting the acquisition of oocyte competence.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-8818
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1262/jrd.2018-080