Preventive effect of mesenchymal stem cell culture supernatant on luminal stricture after endoscopic submucosal dissection in the rectum of pigs

Tsuda, Momoko; Ohnishi, Shunsuke; Mizushima, Takeshi; Hosono, Hidetaka; Yamahara, Kenichi; Ishikawa, Marin; Abiko, Satoshi; Katsurada, Takehiko; Shimizu, Yuichi; Sakamoto, Naoya

Preventive effect of mesenchymal stem cell culture supernatant on luminal stricture after endoscopic submucosal dissection in the rectum of pigs

Tsuda, Momoko; Ohnishi, Shunsuke; Mizushima, Takeshi et al.

Endoscopy, 2018, 50(10), 1001-1016

Permalink : https://hdl.handle.net/2115/73707

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アクセス権	open access
URI	https://hdl.handle.net/2115/73707
タイトル	Preventive effect of mesenchymal stem cell culture supernatant on luminal stricture after endoscopic submucosal dissection in the rectum of pigs
著者	Tsuda, Momoko ; Ohnishi, Shunsuke ; Mizushima, Takeshi ; Hosono, Hidetaka ; Yamahara, Kenichi ; Ishikawa, Marin ; Abiko, Satoshi ; Katsurada, Takehiko ; Shimizu, Yuichi ; Sakamoto, Naoya
著者名 Tsuda, Momoko
e-Rad_Researcher 10443475 著者名 Ohnishi, Shunsuke
著者名 Mizushima, Takeshi
著者名 Hosono, Hidetaka
e-Rad_Researcher 50450888 著者名 Yamahara, Kenichi
著者名 Ishikawa, Marin
著者名 Abiko, Satoshi
著者名 Katsurada, Takehiko
e-Rad_Researcher 90333608 著者名 Shimizu, Yuichi
e-Rad_Researcher 10334418 著者名 Sakamoto, Naoya
言語	英語
発行日	2018-10
出版者	Georg Thieme Verlag
収録物名	Endoscopy
巻(号)	50(10)
ページ	1001-1016
抄録	Background: Mesenchymal stem cells (MSCs) are valuable in regenerative medicine, and MSC culture supernatant (MSC-CS) reportedly inhibits inflammation and fibrosis. We investigated whether colorectal luminal stricture develops after circumferential endoscopic submucosal dissection (ESD) in the colorectum, and whether the development of luminal stricture could be prevented by using MSC-CS enema. Methods: In the first experiment, we performed circumferential ESD in the rectums or distal colons of pigs (n = 4 in each group). We sacrificed the pigs on Day 22 and measured the degree of luminal stricture. In the second experiment, we performed circumferential ESD in the rectums of pigs and administered an MSC-CS gel or a control gel enema after ESD for 4 days. We sacrificed the pigs on Day 8 (n = 3 in each group) or 22 (n = 3 in each group) to measure the degree of luminal stricture, and performed histological analysis. Results: Severe luminal stricture was observed in the rectum but not in the distal colon. Moreover, fiber accumulation in the submucosa and hypertrophy of the muscularis propria were observed in the rectum but not in the distal colon. The degree of luminal stricture in the rectum was significantly lower in the MSC-CS group than in the control group. Furthermore, MSC-CS attenuated myofibroblast activation and hypertrophy of the muscularis propria on Day 22, and reduced inflammatory cell infiltration on Day 8. Conclusions: Luminal stricture after ESD developed only in the rectum because of the difference in myofibroblast activation and fiber accumulation. In addition, MSC-CS enema prevented luminal stricture after ESD, possibly by inhibiting the inflammatory reaction and fibrosis.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is an Accepted Manuscript of an article published by Thieme Publishing Group in Endoscopy on October 2018, available online at https://www.thieme-connect.de/products/ejournals/abstract/10.1055/a-0584-7262
PISSN	0013-726X
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1055/a-0584-7262