Brain-derived neurotrophic factor induces angiogenin secretion and nuclear translocation in human umbilical vein endothelial cells

Mori, Ayako; Nishioka, Yusuke; Yamada, Mai; Nishibata, Yuka; Masuda, Sakiko; Tomaru, Utano; Honma, Naoyuki; Moriyama, Takanori; Ishizu, Akihiro

Brain-derived neurotrophic factor induces angiogenin secretion and nuclear translocation in human umbilical vein endothelial cells

Mori, Ayako; Nishioka, Yusuke; Yamada, Mai et al.

Pathology - Research and Practice, 2018, 214(4), 521-526

Permalink : https://hdl.handle.net/2115/73833

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/73833
タイトル	Brain-derived neurotrophic factor induces angiogenin secretion and nuclear translocation in human umbilical vein endothelial cells
著者	Mori, Ayako ; Nishioka, Yusuke ; Yamada, Mai ; Nishibata, Yuka ; Masuda, Sakiko ; Tomaru, Utano ; Honma, Naoyuki ; Moriyama, Takanori ; Ishizu, Akihiro
著者名 Mori, Ayako
著者名 Nishioka, Yusuke
著者名 Yamada, Mai
著者名 Nishibata, Yuka
著者名 Masuda, Sakiko
e-Rad_Researcher 20360901 著者名 Tomaru, Utano
著者名 Honma, Naoyuki
e-Rad_Researcher 20312423 著者名 Moriyama, Takanori
e-Rad_Researcher 60321957 著者名 Ishizu, Akihiro
言語	英語
キーワード	BDNF
	VEGF
	Angiogenin
	Angiogenesis
発行日	2018-04
出版者	Elsevier
収録物名	Pathology - Research and Practice
巻(号)	214(4)
ページ	521-526
抄録	Brain-derived neurotrophic factor (BDNF) is a well-known humoral protein that induces growth of neurons. Recent studies have suggested that BDNF could act as an angiogenesis inducer similar to vascular endothelial growth factor (VEGF). Angiogenin is a strong mediator of angiogenesis. It has particular characteristics both as a secreted protein and a transcription factor. After being incorporated into the cytoplasm, angiogenin is immediately transferred to the nucleus and then mediates the angiogenic effects of angiogenesis inducers, including VEGF. The aim of this study is to determine the association between BDNF and angiogenin. At first, we determined the secretion of angiogenin from human umbilical vein endothelial cells (HUVEC) induced by BDNF with enzyme-linked immunosorbent assay. Next, we determined BDNF-induced nuclear translocation of angiogenin by immunofluorescent staining. In addition, we examined the mRNA expression of angiogenin in HUVEC before and after BDNF stimulation by quantitative reverse transcriptase-polymerase chain reaction. As a result, we noted that BDNF induced angiogenin secretion and nuclear translocation without an increase in the mRNA expression in HUVEC. Furthermore, we demonstrated that BDNF-induced HUVEC proliferation was significantly suppressed when neomycin, a specific inhibitor of nuclear translocation of angiogenin, was administered. These findings indicate that nuclear translocation of angiogenin is critically involved in BDNF-induced proliferation of HUVEC. In conclusion, angiogenin contributes to angiogenesis induced by BDNF.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0344-0338
関連情報	PMID 29573867
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.prp.2018.02.013