Existence of NEU1 sialidase on mouse thymocytes whose natural substrate is CD5

Ochiai, Shigeko; Matsumoto-Mizuno, Tokuko; Kamimura, Daisuke; Murakami, Masaaki; Kobayashi, Miwako; Matsuoka, Ichiro; Ochiai, Hiroshi; Ishida, Hideharu; Kiso, Makoto; Kamimura, Keiko; Koda, Toshiaki

Existence of NEU1 sialidase on mouse thymocytes whose natural substrate is CD5

Ochiai, Shigeko; Matsumoto-Mizuno, Tokuko; Kamimura, Daisuke et al.

Glycobiology, 2018, 28(5), 306-317

Permalink : https://hdl.handle.net/2115/74370

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アクセス権	open access
URI	https://hdl.handle.net/2115/74370
タイトル	Existence of NEU1 sialidase on mouse thymocytes whose natural substrate is CD5
著者	Ochiai, Shigeko ; Matsumoto-Mizuno, Tokuko ; Kamimura, Daisuke ; Murakami, Masaaki ; Kobayashi, Miwako ; Matsuoka, Ichiro ; Ochiai, Hiroshi ; Ishida, Hideharu ; Kiso, Makoto ; Kamimura, Keiko ; Koda, Toshiaki
e-Rad_Researcher 20001878 著者名 Ochiai, Shigeko 著者別名 Kijimoto, Shigeko
著者名 Matsumoto-Mizuno, Tokuko
e-Rad_Researcher 20391922 著者名 Kamimura, Daisuke
e-Rad_Researcher 00250514 著者名 Murakami, Masaaki
e-Rad_Researcher 30396329 著者名 Kobayashi, Miwako
e-Rad_Researcher 40157269 著者名 Matsuoka, Ichiro
e-Rad_Researcher 10002122 著者名 Ochiai, Hiroshi
e-Rad_Researcher 20203002 著者名 Ishida, Hideharu
e-Rad_Researcher 90092931 著者名 Kiso, Makoto
著者名 Kamimura, Keiko
e-Rad_Researcher 20170186 著者名 Koda, Toshiaki
言語	英語
キーワード	CD5
	mRNA of 4 thymus-sialidases
	NEU1 on thymocyte
	NEU1-selective inhibitor
	SM/J mouse
発行日	2018-05
出版者	Oxford University Press
収録物名	Glycobiology
巻(号)	28(5)
ページ	306-317
抄録	Membrane-bound sialidases in the mouse thymus are unique and mysterious because their activity at pH 6.5 is equal to or higher than that in the acidic region. The pH curve like this has never been reported in membrane-bound form. To clarify this enzyme, we studied the sialidase activities of crude membrane fractions from immature-T, mature-T and non-T cells from C57BU6 mice and from SM/J mice, a strain with a defect in NEU1 activity. Non-T cells from C57BL/6 mice had high activity at pH 6.5, but those from SM/J mice did not. Neu1 and Neu3 mRNA was shown by real-time PCR to be expressed in T cells and also in non-T cells, whereas Neu2 was expressed mainly in non-T cells and Neu4 was scarcely expressed. However, the in situ hybridization study on the localization of four sialidases in the thymus showed that Neu4 was clearly expressed. We then focused on a sialidase on the thymocyte surface because the possibility of the existence of a sialidase on thymocytes was suggested by peanut agglutinin (PNA) staining after incubation of the cells alone in PBS. This activity was inhibited by NEU1-selective sialidase inhibitor C9-butyl-amide-2-deoxy-2,3-dehydro-N-acetylneuraminic acid. The natural substrate for the cell surface sialidase was identified as clustered differentiation 5 (CD5) by PNA-blot analysis of anti-CD5 immunoprecipitate. We conclude that NEU1 exists on the cell surface of mouse thymocytes and CD5 is a natural substrate for it. Although this is not the main reaction of the membrane-bound thymus-sialidases, it must be important for the thymus.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	This is a pre-copyedited, author-produced version of an article accepted for publication in Glycobiology following peer review. The version of record Glycobiology, Volume 28, Issue 5, 1 May 2018, Pages 306–317, is available online at: https://academic.oup.com/glycob/article/28/5/306/4829948
PISSN	0959-6658
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1093/glycob/cwy009