A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells

Fujioka, Yoichiro; Nishide, Shinya; Ose, Toyoyuki; Suzuki, Tadaki; Kato, Izumi; Fukuhara, Hideo; Fujioka, Mari; Horiuchi, Kosui; Satoh, Aya O.; Nepal, Prabha; Kashiwagi, Sayaka; Wang, Jing; Horiguchi, Mika; Sato, Yuko; Paudel, Sarad; Nanbo, Asuka; Miyazaki, Tadaaki; Hasegawa, Hideki; Maenaka, Katsumi; Ohba, Yusuke

A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells

Fujioka, Yoichiro; Nishide, Shinya; Ose, Toyoyuki et al.

Cell host & microbe, 2018, 23(6), 809-818

Permalink : https://hdl.handle.net/2115/74619

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/74619
タイトル	A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells
著者	Fujioka, Yoichiro ; Nishide, Shinya ; Ose, Toyoyuki ; Suzuki, Tadaki ; Kato, Izumi ; Fukuhara, Hideo ; Fujioka, Mari ; Horiuchi, Kosui ; Satoh, Aya O. ; Nepal, Prabha ; Kashiwagi, Sayaka ; Wang, Jing ; Horiguchi, Mika ; Sato, Yuko ; Paudel, Sarad ; Nanbo, Asuka ; Miyazaki, Tadaaki ; Hasegawa, Hideki ; Maenaka, Katsumi ; Ohba, Yusuke
著者名 Fujioka, Yoichiro
e-Rad_Researcher 40451398 著者名 Nishide, Shinya
e-Rad_Researcher 80380525 著者名 Ose, Toyoyuki
e-Rad_Researcher 30527180 著者名 Suzuki, Tadaki
著者名 Kato, Izumi
著者名 Fukuhara, Hideo
著者名 Fujioka, Mari
著者名 Horiuchi, Kosui
著者名 Satoh, Aya O.
著者名 Nepal, Prabha
著者名 Kashiwagi, Sayaka
著者名 Wang, Jing
著者名 Horiguchi, Mika
著者名 Sato, Yuko
著者名 Paudel, Sarad
e-Rad_Researcher 60359487 著者名 Nanbo, Asuka
e-Rad_Researcher 60272431 著者名 Miyazaki, Tadaaki
著者名 Hasegawa, Hideki
e-Rad_Researcher 10322752 著者名 Maenaka, Katsumi
e-Rad_Researcher 30333503 著者名 Ohba, Yusuke
言語	英語
キーワード	influenza A virus
	virus entry
	calcium ion
	calcium channel
	calcium channel blockers
	hemagglutinin
	sialylation
	virus-host cell interaction
発行日	2018-06-13
出版者	Cell Press
収録物名	Cell host & microbe
巻(号)	23(6)
ページ	809-818
抄録	Influenza A virus (IAV) infection is initiated by the attachment of the viral glycoprotein hemagglutinin (HA) to sialic acid on the host cell surface. However, the sialic acid-containing receptor crucial for IAV infection has remained unidentified. Here, we show that HA binds to the voltage-dependent Ca2+ channel Cav1.2 to trigger intracellular Ca2+ oscillations and subsequent IAV entry and replication. IAV entry was inhibited by Ca2+ channel blockers (CCBs) or by knockdown of Cav1.2. The CCB diltiazem also inhibited virus replication in vivo. Reintroduction of wild-type but not the glycosylation-deficient mutants of Cav1.2 restored Ca2+ oscillations and virus infection in Cav1.2-depleted cells, demonstrating the significance of Cav1.2 sialylation. Taken together, we identify Cav1.2 as a sialylated host cell surface receptor that binds HA and is critical for IAV entry.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1931-3128
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.chom.2018.04.015