Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

Takehana, Kazuya; Kinjyo, Teruo; Nemoto, Manabu; Matsuno, Keita

Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)

Takehana, Kazuya; Kinjyo, Teruo; Nemoto, Manabu et al.

Journal of veterinary medical science, 2019, 81(3), 504-507

Permalink : https://hdl.handle.net/2115/74932

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アクセス権	open access
URI	https://hdl.handle.net/2115/74932
タイトル	Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP)
著者	Takehana, Kazuya ; Kinjyo, Teruo ; Nemoto, Manabu ; Matsuno, Keita
著者名 Takehana, Kazuya
著者名 Kinjyo, Teruo
著者名 Nemoto, Manabu
e-Rad_Researcher 40753306 著者名 Matsuno, Keita
言語	英語
キーワード	Acute hemorrhagic disease
	Asian elephants (Elephas maximus)
	Elephant endotheliotropic herpesvirus (EEHV)
	Loop-mediated isothermal amplification (LAMP)
	Polymerase chain reaction (PCR)
発行日	2019-03
出版者	公益社団法人日本獣医学会
出版者	The Japanese Society of Veterinary Science
収録物名	Journal of veterinary medical science
巻(号)	81(3)
ページ	504-507
抄録	Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 101.2 copies/mu l and 100-fold higher than that of conventional PCR. These advantages of the LAMP assay for EEHV1 detection may facilitate better veterinary practices for treating elephants suffering from the acute disease.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0916-7250
EISSN	1347-7439
NCID	AA10796138
関連情報	PMID 30700652
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1292/jvms.18-0683