Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells

Yanagawa-Matsuda, Aya; Mikawa, Yohei; Habiba, Umma; Kitamura, Tetsuya; Yasuda, Motoaki; Towfik-Alam, Mohammad; Kitagawa, Yoshimasa; Minowa, Kazuyuki; Shindoh, Masanobu; Higashino, Fumihiro

Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells

Yanagawa-Matsuda, Aya; Mikawa, Yohei; Habiba, Umma et al.

Oncology reports, 2019, 41(2), 954-960

Permalink : https://hdl.handle.net/2115/75080

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アクセス権	open access
URI	https://hdl.handle.net/2115/75080
タイトル	Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells
著者	Yanagawa-Matsuda, Aya ; Mikawa, Yohei ; Habiba, Umma ; Kitamura, Tetsuya ; Yasuda, Motoaki ; Towfik-Alam, Mohammad ; Kitagawa, Yoshimasa ; Minowa, Kazuyuki ; Shindoh, Masanobu ; Higashino, Fumihiro
e-Rad_Researcher 60514312 著者名 Yanagawa-Matsuda, Aya
著者名 Mikawa, Yohei
著者名 Habiba, Umma
e-Rad_Researcher 00451451 著者名 Kitamura, Tetsuya
e-Rad_Researcher 90239765 著者名 Yasuda, Motoaki
e-Rad_Researcher 60641694 著者名 Towfik-Alam, Mohammad
e-Rad_Researcher 00224957 著者名 Kitagawa, Yoshimasa
e-Rad_Researcher 30209845 著者名 Minowa, Kazuyuki
e-Rad_Researcher 20162802 著者名 Shindoh, Masanobu
e-Rad_Researcher 50301891 著者名 Higashino, Fumihiro
言語	英語
キーワード	adenovirus
	AU-rich element
	ARE-mRNA
	HuR
	E4orf6
発行日	2019-02
出版者	Spandidos Publications
収録物名	Oncology reports
巻(号)	41(2)
ページ	954-960
抄録	AU-rich elements (AREs) are RNA elements that enhance the rapid decay of mRNA. The fate of ARE-mRNA is controlled by ARE-binding proteins. HuR, a member of the embryonic lethal abnormal vision (ELAV) family of RNA-binding proteins, is involved in the export and stabilization of ARE-mRNA. In the vast majority of cancer cells, HuR constitutively relocates to the cytoplasm, resulting in the stabilization of ARE-mRNA. Previously, we described that the adenovirus gene product, E4orf6, which is necessary for virus replication, participates in ARE-mRNA export and stabilization. In the present study, we showed the oncolytic potential of E4orf6-deleted adenovirus dl355, which is expected to be replicated selectively in cancer cells. Virus production and cytolytic activity of dl355 were higher in cancer cells than in normal cells. HuR-depletion downregulated dl355 replication, demonstrating that ARE-mRNA stabilization is required for the production of this virus. Tumor growth was inhibited in nude mice by an intratumoral injection of dl355. Furthermore, dl355 had a stronger oncolytic effect than E1B55k-deleted adenovirus. These results indicate that dl355 has potential as an oncolytic adenovirus for a large number of cancers where ARE-mRNA is stabilized.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
PISSN	1021-335X
関連情報	PMID 30431137
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3892/or.2018.6865