Interaction of Nerve Growth Factor beta with Adiponectin and SPARC Oppositely Modulates its Biological Activity

Okura, Yuu; Imao, Takeshi; Murashima, Seisuke; Shibata, Haruki; Kamikavwa, Akihiro; Okamatsu-Ogura, Yuko; Saito, Masayuki; Kimura, Kazuhiro

Interaction of Nerve Growth Factor beta with Adiponectin and SPARC Oppositely Modulates its Biological Activity

Okura, Yuu; Imao, Takeshi; Murashima, Seisuke et al.

International Journal of Molecular Sciences, 2019, 20(7), 1541

Permalink : https://hdl.handle.net/2115/75164

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アクセス権	open access
URI	https://hdl.handle.net/2115/75164
タイトル	Interaction of Nerve Growth Factor beta with Adiponectin and SPARC Oppositely Modulates its Biological Activity
著者	Okura, Yuu ; Imao, Takeshi ; Murashima, Seisuke ; Shibata, Haruki ; Kamikavwa, Akihiro ; Okamatsu-Ogura, Yuko ; Saito, Masayuki ; Kimura, Kazuhiro
著者名 Okura, Yuu
著者名 Imao, Takeshi
著者名 Murashima, Seisuke
著者名 Shibata, Haruki
著者名 Kamikavwa, Akihiro
著者名 Okamatsu-Ogura, Yuko
著者名 Saito, Masayuki
e-Rad_Researcher 30192561 著者名 Kimura, Kazuhiro
言語	英語
キーワード	adiponectin
	AMPK
	BIAcore
	extracellular signal-regulated kinase (ERK)
	matricellular proteins
	neuritogenesis
	NGF beta
	PC12 cells
	Secreted protein
	acidic and rich in cysteine (SPARC)
発行日	2019-03-27
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	20(7)
ページ	1541
抄録	Both adiponectin and secreted protein, acidic and rich in cysteine (SPARC) inhibit platelet-derived growth factor-BB (PDGF-BB)-induced and basic fibroblast growth factor (FGF2)-induced angiogenic activities through direct and indirect interactions. Although SPARC enhances nerve growth factor (NGF)-dependent neurogenesis, the physical interaction of NGF beta with adiponectin and SPARC remains obscure. Therefore, we first examined their intermolecular interaction by surface plasmon resonance method. NGF beta bound to immobilized SPARC with the binding constant of 59.4 nM, comparable with that of PDGF-BB (24.5 nM) but far less than that of FGF2 (14.4 mu M). NGF beta bound to immobilized full length adiponectin with the binding constant of 103 nM, slightly higher than those of PDGF-BB (24.3 nM) and FGF2 (80.2 nM), respectively. Treatment of PC12 cells with SPARC did not cause mitogen-activated protein kinase (MAPK) activation and neurite outgrowth. However, simultaneous addition of SPARC with NGF beta enhanced NGF beta-induced MAPK phosphorylation and neurite outgrowth. Treatment of the cells with adiponectin increased AMP-activated protein kinase (AMPK) phosphorylation but failed to induce neurite outgrowth. Simultaneous treatment with NGF beta and adiponectin significantly reduced cell size and the number of cells with neurite, even after silencing the adiponectin receptors by their siRNA. These results indicate that NGF beta directly interacts with adiponectin and SPARC, whereas these interactions oppositely regulate NGF beta functions.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1422-0067
関連情報	PMID 30934765
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms20071541