Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses

Ishii, Hirokazu; Otomo, Kohei; Hung, Jui-Hung; Tsutsumi, Motosuke; Yokoyama, Hiroyuki; Nemoto, Tomomi

Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses

Ishii, Hirokazu; Otomo, Kohei; Hung, Jui-Hung et al.

Biomedical Optics Express, 2019, 10(7), 3104-3113

Permalink : https://hdl.handle.net/2115/75168

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アクセス権	open access
URI	https://hdl.handle.net/2115/75168
タイトル	Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses
著者	Ishii, Hirokazu ; Otomo, Kohei ; Hung, Jui-Hung ; Tsutsumi, Motosuke ; Yokoyama, Hiroyuki ; Nemoto, Tomomi
著者名 Ishii, Hirokazu
e-Rad_Researcher 40547204 著者名 Otomo, Kohei
著者名 Hung, Jui-Hung
e-Rad_Researcher 60782422 著者名 Tsutsumi, Motosuke
著者名 Yokoyama, Hiroyuki
e-Rad_Researcher 50291084 著者名 Nemoto, Tomomi
言語	英語
発行日	2019-07-01
出版者	Optical Society of America
収録物名	Biomedical Optics Express
巻(号)	10(7)
ページ	3104-3113
抄録	We developed two-photon excitation stimulated emission depletion (STED) nanoscopy using high-peak-power sub-nanosecond 655-nm pulses. The STED pulse exhibited ideal optical properties and sufficient pulse energy to realize a 70-nm spatial resolution in the compact setup With electrically controllable components. For biological applications, we screened suitable fluorescent dyes or proteins and realized the sub-100 nm spatial resolution imaging of presynaptic protein clusters in fixed primary cultured neurons Without severe photobleaching. We expect this method to enable visualization of ultrastructures and the cluster dynamics of biomolecules representing physiological functions in living cells and tissue. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	© 2019 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
PISSN	2156-7085
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1364/BOE.10.003104