HuR translocation to the cytoplasm of cancer cells in actin-independent manner

Habiba, Umma; Kuroshima, Takeshi; Yanagawa-Matsuda, Aya; Kitamura, Tetsuya; Chowdhury, A. F. M. A.; Jehung, Jumond P.; Hossain, Elora; Sano, Hidehiko; Kitagawa, Yoshimasa; Shindoh, Masanobu; Higashino, Fumihiro

HuR translocation to the cytoplasm of cancer cells in actin-independent manner

Habiba, Umma; Kuroshima, Takeshi; Yanagawa-Matsuda, Aya et al.

Experimental cell research, 2018, 369(2), 218-225

Permalink : https://hdl.handle.net/2115/75223

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アクセス権	open access
URI	https://hdl.handle.net/2115/75223
タイトル	HuR translocation to the cytoplasm of cancer cells in actin-independent manner
著者	Habiba, Umma ; Kuroshima, Takeshi ; Yanagawa-Matsuda, Aya ; Kitamura, Tetsuya ; Chowdhury, A. F. M. A. ; Jehung, Jumond P. ; Hossain, Elora ; Sano, Hidehiko ; Kitagawa, Yoshimasa ; Shindoh, Masanobu ; Higashino, Fumihiro
著者名 Habiba, Umma
著者名 Kuroshima, Takeshi
e-Rad_Researcher 60514312 著者名 Yanagawa-Matsuda, Aya
e-Rad_Researcher 00451451 著者名 Kitamura, Tetsuya
著者名 Chowdhury, A. F. M. A.
著者名 Jehung, Jumond P.
著者名 Hossain, Elora
e-Rad_Researcher 90205998 著者名 Sano, Hidehiko
e-Rad_Researcher 00224957 著者名 Kitagawa, Yoshimasa
e-Rad_Researcher 20162802 著者名 Shindoh, Masanobu
e-Rad_Researcher 50301891 著者名 Higashino, Fumihiro
言語	英語
キーワード	HuR, human antigen R
	ARE, AU-rich element
	3 '-untranslated region (3 '-UTR)
	Latrunculin A (LatA)
	Blebbistatin (blebbistat)
	Human Angiotensin II (Ang.II)
	COX-2, cyclooxygenase-2
発行日	2018-08-15
出版者	Elsevier
収録物名	Experimental cell research
巻(号)	369(2)
ページ	218-225
抄録	Human antigen R (HuR) is a RNA-binding protein, which binds to the AU-rich element (ARE) in the 3'-untranslated region (3'-UTR) of certain mRNA and is involved in the export and stabilization of ARE-mRNA. HuR constitutively relocates to the cytoplasm in many cancer cells, however the mechanism of intracellular HuR trafficking is poorly understood. To address this question, we examined the functional role of the cytoskeleton in HuR relocalization. We tested the effect of actin depolymerizing macrolide latrunculin A or myosin II ATPase activity inhibitor blebbistatin for HuR relocalization induced by the vasoactive hormone Angiotensin II in cancer and control normal cells. Western blot and confocal imaging data revealed that both inhibitors attenuated the cytoplasmic HuR in normal cells but no such alteration was observed in cancer cells. Concomitant with changes in intracellular HuR localization, both inhibitors markedly decreased the accumulation and half-lives of HuR target ARE-mRNAs in normal cells, whereas no change was observed in cancer cells. Furthermore, co-immunoprecipitation experiments with HuR proteins revealed clear physical interaction with beta-actin only in normal cells. The current study is the first to verify that cancer cells can implicate a microfilament independent HuR transport. We hypothesized that when cytoskeleton structure is impaired, cancer cells can acquire an alternative HuR trafficking strategy.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0014-4827
NCID	AA00641011
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.yexcr.2018.05.021