Investigation of mRNA expression changes associated with field exposure to DDTs in chickens from KwaZulu-Natal, South Africa

Thompson, Lesa A.; Ikenaka, Yoshinori; Darwish, Wageh S.; Yohannes, Yared B.; van Vuren, Johan J.; Wepener, Victor; Smit, Nico J.; Assefa, Atnafu G.; Tharwat, Ahmed; Eldin, Walaa Fathy Saad; Nakayama, Shouta M. M.; Mizukawa, Hazuki; Ishizuka, Mayumi

Investigation of mRNA expression changes associated with field exposure to DDTs in chickens from KwaZulu-Natal, South Africa

Thompson, Lesa A.; Ikenaka, Yoshinori; Darwish, Wageh S. et al.

PLoS ONE, 2018, 13(10), e0204400

Permalink : https://hdl.handle.net/2115/75302

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アクセス権	open access
URI	https://hdl.handle.net/2115/75302
タイトル	Investigation of mRNA expression changes associated with field exposure to DDTs in chickens from KwaZulu-Natal, South Africa
著者	Thompson, Lesa A. ; Ikenaka, Yoshinori ; Darwish, Wageh S. ; Yohannes, Yared B. ; van Vuren, Johan J. ; Wepener, Victor ; Smit, Nico J. ; Assefa, Atnafu G. ; Tharwat, Ahmed ; Eldin, Walaa Fathy Saad ; Nakayama, Shouta M. M. ; Mizukawa, Hazuki ; Ishizuka, Mayumi
著者名 Thompson, Lesa A.
著者名 Ikenaka, Yoshinori
著者名 Darwish, Wageh S.
著者名 Yohannes, Yared B.
著者名 van Vuren, Johan J.
著者名 Wepener, Victor
著者名 Smit, Nico J.
著者名 Assefa, Atnafu G.
著者名 Tharwat, Ahmed
著者名 Eldin, Walaa Fathy Saad
著者名 Nakayama, Shouta M. M.
著者名 Mizukawa, Hazuki
e-Rad_Researcher 50332474 著者名 Ishizuka, Mayumi
言語	英語
発行日	2018-10-11
出版者	PLOS
収録物名	PLoS ONE
巻(号)	13(10)
ページ	e0204400
抄録	The objective of this study was to identify potential mRNA expression changes in chicken livers associated with environmental exposure to dichloro-diphenyl-trichloroethane (DDT) and its metabolites (DDTs). In particular, we focused on genes relating to the immune system and metabolism. We analyzed liver samples from free-ranging chickens in KwaZulu-Natal, South Africa, for contamination by DDTs. This area predominantly uses DDT in its malaria control program, and homes are sprayed annually with the pesticide. Genes relating to the immune system and metabolism were selected as potential genetic biomarkers that could be linked to higher contamination with DDTs. RT-qPCR analysis on 39 samples showed strong correlations between DDTs contamination and mRNA expression for the following genes: AvBD1, AvBD2, AvBD6 and AvBD7(down-regulated), and CYP17, ELOVL2 and SOLE (up-regulated). This study shows for the first time interesting and significant correlations between genetic material collected from environmentally-exposed chickens and mRNA expression of several genes involved in immunity and metabolism. These findings show the usefulness of analysis on field samples from a region with high levels of environmental contamination in detecting potential biomarkers of exposure. In particular, we observed clear effects from DDT contamination on mRNA expression of genes involved in immune suppression, endocrine-disrupting effects, and lipid dysregulation. These results are of interest in guiding future studies to further elucidate the pathways involved in and clinical importance of toxicity associated with DDT exposure from contaminated environments, to ascertain the health risk to livestock and any subsequent risks to food security for people.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
PISSN	1932-6203
関連情報	PMID 30307967
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0204400