Validation of the use of an artificial mitochondrial reporter DNA vector containing a Cytomegalovirus promoter for mitochondrial transgene expression

Yamada, Yuma; Ishikawa, Takuya; Harashima, Hideyoshi

Validation of the use of an artificial mitochondrial reporter DNA vector containing a Cytomegalovirus promoter for mitochondrial transgene expression

Yamada, Yuma; Ishikawa, Takuya; Harashima, Hideyoshi

Biomaterials, 2017, 136, 56-66

Permalink : https://hdl.handle.net/2115/75315

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論文情報

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アクセス権	open access
URI	https://hdl.handle.net/2115/75315
タイトル	Validation of the use of an artificial mitochondrial reporter DNA vector containing a Cytomegalovirus promoter for mitochondrial transgene expression
著者	Yamada, Yuma ; Ishikawa, Takuya ; Harashima, Hideyoshi
e-Rad_Researcher 60451431 著者名 Yamada, Yuma
著者名 Ishikawa, Takuya
e-Rad_Researcher 00183567 著者名 Harashima, Hideyoshi
言語	英語
キーワード	Mitochondria
	Transgene expression
	CMV promotor
	Mitochondrial delivery
	Hydrodynamic injection
発行日	2017-08
出版者	Elsevier
収録物名	Biomaterials
巻(号)	136
ページ	56-66
抄録	Mitochondria have their own gene expression system that is independent of the nuclear system, and control cellular functions in cooperation with the nucleus. While a number of useful technologies for achieving nuclear transgene expression have been reported, only a few have focused on mitochondria. In this study, we validated the utility of an artificial mitochondrial DNA vector with a virus promoter on mitochondrial transgene expression. We designed and constructed pCMV-mtLuc (CGG) that contains a CMV promotor derived from Cytomegalovirus and an artificial mitochondrial genome with a NanoLuc (Nluc) luciferase gene that records adjustments to the mitochondrial codon system. Nluc luciferase activity measurements showed that the pCMV-mtLuc (CGG) efficiently produced the Nluc luciferase protein in human HeLa cells. Moreover, we optimized the mitochondrial transfection of pCMV-mtLuc (CGG) using a MITO-Porter system, a liposome-based carrier for mitochondrial delivery via membrane fusion. As a result, we found that transfection of pCMV-mtLuc (CGG) by MITO-Porter modified with the KALA peptide (cationic amphipathic cell-penetrating peptide) showed a high mitochondrial transgene expression. The developed mitochondrial transgene expression system represents a potentially useful tool for the fields of nanoscience and nanotechnology for controlling the intracellular microenvironment via the regulation of mitochondrial function and promises to open additional innovative research fields of study. (C) 2017 Elsevier Ltd. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0142-9612
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.biomaterials.2017.05.016