open access

https://hdl.handle.net/2115/75320

Isolation and lipolytic activity of eurycomanone and its epoxy derivative from Eurycoma longifolia

英語

Quassinoid

2017-09-01

Elsevier

Bioorganic and Medicinal Chemistry

25(17)

4829-4834

Eurycomanone (1) and 13β,21-epoxyeurycomanone (2) were isolated from Eurycoma longifolia for studies of lipolytic activity. Compound 1 enhanced lipolysis in adipocytes with an EC₅₀ of 14.6μM, while its epoxy derivate, compound 2, had a stronger activity with an EC₅₀ of 8.6μM. Based on molecular mechanistic study using several specific inhibitors to lipolytic signaling pathways, it was found that PKA inhibitor totally diminished the lipolytic activity of 1 and 2. Further immunoblotting analysis confirmed the activation of phosphorylated PKA by both 1 and 2. With the growing need to develop new anti-obesity agents, eurycomanone and its epoxy derivate can be used as promising lead compounds to target lipid catabolism.

学術雑誌論文

Accepted Manuscript

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

© 2017, Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

0968-0896

1464-3391

AA10938083