Identification of RNA aptamer which specifically interacts with PtdIns(3)P

Donia, Thoria; Jyoti, Bala; Suizu, Futoshi; Hirata, Noriyuki; Tanaka, Tsutomu; Ishigaki, Satoko; Thomas, Pranzatelli J. F.; Nio-Kobayashi, Junko; Iwanaga, Toshihiko; Chiorini, John A.; Noguchi, Masayuki

Identification of RNA aptamer which specifically interacts with PtdIns(3)P

Donia, Thoria; Jyoti, Bala; Suizu, Futoshi et al.

Biochemical and biophysical research communications, 2019, 517(1), 146-154

Permalink : https://hdl.handle.net/2115/75448

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アクセス権	open access
URI	https://hdl.handle.net/2115/75448
タイトル	Identification of RNA aptamer which specifically interacts with PtdIns(3)P
著者	Donia, Thoria ; Jyoti, Bala ; Suizu, Futoshi ; Hirata, Noriyuki ; Tanaka, Tsutomu ; Ishigaki, Satoko ; Thomas, Pranzatelli J. F. ; Nio-Kobayashi, Junko ; Iwanaga, Toshihiko ; Chiorini, John A. ; Noguchi, Masayuki
著者名 Donia, Thoria
著者名 Jyoti, Bala
e-Rad_Researcher 90431379 著者名 Suizu, Futoshi
著者名 Hirata, Noriyuki
著者名 Tanaka, Tsutomu
著者名 Ishigaki, Satoko
著者名 Thomas, Pranzatelli J. F.
e-Rad_Researcher 70447043 著者名 Nio-Kobayashi, Junko
e-Rad_Researcher 10160128 著者名 Iwanaga, Toshihiko
著者名 Chiorini, John A.
e-Rad_Researcher 40359477 著者名 Noguchi, Masayuki
言語	英語
キーワード	Phosphoinositide
	RNA aptamer
	Autophagy
	Molecular imaging
	PI3K
	Lysosome
発行日	2019-09-10
出版者	Elsevier
収録物名	Biochemical and biophysical research communications
巻(号)	517(1)
ページ	146-154
抄録	The phosphinositide Ptdlns(3)P plays an important role in autophagy; however, the detailed mechanism of its activity remains unclear. Here, we used a Systematic Evolution of Ligands by EXponential enrichment (SELEX) screening approach to identify an RNA aptamer of 40 nucleotides that specifically recognizes and binds to intracellular lysosomal Ptdlns(3)P. Binding occurs in a magnesium concentration- and pH-dependent manner, and consequently inhibits autophagy as determined by LC3II/I conversion, p62 degradation, formation of LC3 puncta, and lysosomal accumulation of Phafin2. These effects in turn inhibited lysosomal acidification, and the subsequent hydrolytic activity of cathepsin D following induction of autophagy. Given the essential role of Ptdlns(3)P as a key targeting molecule for autophagy induction, identification of this novel Ptdlns(3)P RNA aptamer provides new opportunities for investigating the biological functions and mechanisms of phosphoinositides. (C) 2019 The Authors. Published by Elsevier Inc.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN	0006-291X
関連情報	PMID 31351587
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1016/j.bbrc.2019.07.034