Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes

Homan, Kentaro; Hanamatsu, Hisatoshi; Furukawa, Jun-ichi; Okada, Kazue; Yokota, Ikuko; Onodera, Tomohiro; Iwasaki, Norimasa

Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes

Homan, Kentaro; Hanamatsu, Hisatoshi; Furukawa, Jun-ichi et al.

International Journal of Molecular Sciences, 2019, 20(14), 3546

Permalink : https://hdl.handle.net/2115/75510

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https://doi.org/10.3390/ijms20143546			36

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アクセス権	open access
URI	https://hdl.handle.net/2115/75510
タイトル	Alteration of the Total Cellular Glycome during Late Differentiation of Chondrocytes
著者	Homan, Kentaro ; Hanamatsu, Hisatoshi ; Furukawa, Jun-ichi ; Okada, Kazue ; Yokota, Ikuko ; Onodera, Tomohiro ; Iwasaki, Norimasa
e-Rad_Researcher 40823331 著者名 Homan, Kentaro
e-Rad_Researcher 70734185 著者名 Hanamatsu, Hisatoshi
e-Rad_Researcher 30374193 著者名 Furukawa, Jun-ichi
著者名 Okada, Kazue
著者名 Yokota, Ikuko
e-Rad_Researcher 70547174 著者名 Onodera, Tomohiro
e-Rad_Researcher 30322803 著者名 Iwasaki, Norimasa
言語	英語
キーワード	chondrocyte hypertrophy
	glycomics
	glycoconjugate
	glycosphingolipid
	glycosaminoglycan
発行日	2019-07-02
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	20(14)
ページ	3546
抄録	In normal articular cartilage, chondrocytes do not readily proliferate or terminally differentiate, and exhibit a low level of metabolism. Hypertrophy-like changes of chondrocytes have been proposed to play a role in the pathogenesis of osteoarthritis by inducing protease-mediated cartilage degradation and calcification; however, the molecular mechanisms underlying these changes are unclear. Glycans are located on the outermost cell surface. Dynamic cellular differentiation can be monitored and quantitatively characterized by profiling the glycan structures of total cellular glycoproteins. This study aimed to clarify the alterations in glycans upon late differentiation of chondrocytes, during which hypertrophy-like changes occur. Primary mouse chondrocytes were differentiated using an insulin-induced chondro-osteogenic differentiation model. Comprehensive glycomics, including N-glycans, O-glycans, free oligosaccharides, glycosaminoglycan, and glycosphingolipid, were analyzed for the chondrocytes after 0-, 10- and 20-days cultivation. The comparison and clustering of the alteration of glycans upon hypertrophy-like changes of primary chondrocytes were performed. Comprehensive glycomic analyses provided complementary alterations in the levels of various glycans derived from glycoconjugates during hypertrophic differentiation. In addition, expression of genes related to glycan biosynthesis and metabolic processes was significantly correlated with glycan alterations. Our results indicate that total cellular glycan alterations are closely associated with chondrocyte hypertrophy and help to describe the glycophenotype by chondrocytes and their hypertrophic differentiation. our results will assist the identification of diagnostic and differentiation biomarkers in the future.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
権利情報	© 2019 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
PISSN	1661-6596
関連情報	PMID 31331074
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.3390/ijms20143546