Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses

Sakurai, Akira; Takayama, Katsuyoshi; Nomura, Namiko; Kajiwara, Naoki; Okamatsu, Masatoshi; Yamamoto, Naoki; Tamura, Tsuruki; Yamada, Jitsuho; Hashimoto, Masako; Sakoda, Yoshihiro; Suda, Yoshihiko; Kobayashi, Yukuharu; Kida, Hiroshi; Shibasaki, Futoshi

Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses

Sakurai, Akira; Takayama, Katsuyoshi; Nomura, Namiko et al.

PLoS One, 2015, 10(2), e0116715

Permalink : https://hdl.handle.net/2115/75587

このアイテムのアクセス数:621件（2026-08-11 04:55 集計）

閲覧可能ファイル

ファイル	フォーマット	サイズ	閲覧回数	説明
https://doi.org/10.1371/journal.pone.0116715			19

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

アクセス権	open access
URI	https://hdl.handle.net/2115/75587
タイトル	Fluorescent immunochromatography for rapid and sensitive typing of seasonal influenza viruses
著者	Sakurai, Akira ; Takayama, Katsuyoshi ; Nomura, Namiko ; Kajiwara, Naoki ; Okamatsu, Masatoshi ; Yamamoto, Naoki ; Tamura, Tsuruki ; Yamada, Jitsuho ; Hashimoto, Masako ; Sakoda, Yoshihiro ; Suda, Yoshihiko ; Kobayashi, Yukuharu ; Kida, Hiroshi ; Shibasaki, Futoshi
著者名 Sakurai, Akira
著者名 Takayama, Katsuyoshi
e-Rad_Researcher 50599694 著者名 Nomura, Namiko
e-Rad_Researcher 70453917 著者名 Kajiwara, Naoki
e-Rad_Researcher 00507163 著者名 Okamatsu, Masatoshi
著者名 Yamamoto, Naoki
著者名 Tamura, Tsuruki
著者名 Yamada, Jitsuho
著者名 Hashimoto, Masako
e-Rad_Researcher 40333637 著者名 Sakoda, Yoshihiro
著者名 Suda, Yoshihiko
著者名 Kobayashi, Yukuharu
e-Rad_Researcher 10109506 著者名 Kida, Hiroshi
e-Rad_Researcher 90300954 著者名 Shibasaki, Futoshi
言語	英語
発行日	2015-02-04
出版者	Public Library of Science
収録物名	PLoS One
巻(号)	10(2)
ページ	e0116715
抄録	Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per reaction. Recently, we reported an improved IC assay using antibodies conjugated with fluorescent beads (fluorescent immunochromatography; FLIC) for subtyping H5 influenza viruses (FLIC-H5). Although the FLIC strip must be scanned using a fluorescent reader, the sensitivity (LOD) is significantly improved over that of conventional IC methods. In addition, the antibodies which are specific against the subtypes of influenza viruses cannot be available for the detection of other subtypes when the major antigenicity will be changed. In this study, we established the use of FLIC to type seasonal influenza A and B viruses (FLIC-AB). This method has improved sensitivity to 100-fold higher than that of conventional IC methods when we used several strains of influenza viruses. In addition, FLIC-AB demonstrated the ability to detect influenza type A and influenza type B viruses from clinical samples with high sensitivity and specificity (Type A: sensitivity 98.7% (74/75), specificity 100% (54/54), Type B: sensitivity 100% (90/90), specificity 98.2% (54/55) in nasal swab samples) in comparison to the results of qRT-PCR. And furthermore, FLIC-AB performs better in the detection of early stage infection (under 13h) than other conventional IC methods. Our results provide new strategies to prevent the early-stage transmission of influenza viruses in humans during both seasonal outbreaks and pandemics.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution 4.0 International
EISSN	1932-6203
関連情報	PMID 25650570
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1371/journal.pone.0116715