Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells.

Furugen, Ayako; Ishiguro, Yuri; Kobayashi, Masaki; Narumi, Katsuya; Nishimura, Ayako; Hirano, Takeshi; Iseki, Ken

Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells.

Furugen, Ayako; Ishiguro, Yuri; Kobayashi, Masaki et al.

Reproductive toxicology (Elmsford, N.Y.), 2017, 67, 48-55

Permalink : https://hdl.handle.net/2115/75610

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アクセス権	open access
URI	https://hdl.handle.net/2115/75610
タイトル	Involvement of l-type amino acid transporter 1 in the transport of gabapentin into human placental choriocarcinoma cells.
著者	Furugen, Ayako ; Ishiguro, Yuri ; Kobayashi, Masaki ; Narumi, Katsuya ; Nishimura, Ayako ; Hirano, Takeshi ; Iseki, Ken
e-Rad_Researcher 90767261 著者名 Furugen, Ayako
著者名 Ishiguro, Yuri
e-Rad_Researcher 70431319 著者名 Kobayashi, Masaki
e-Rad_Researcher 90746752 著者名 Narumi, Katsuya
著者名 Nishimura, Ayako
著者名 Hirano, Takeshi
e-Rad_Researcher 40203062 著者名 Iseki, Ken
言語	英語
キーワード	BeWo cells
	Gabapentin (GBP)
	JEG-3 cells
	Organic cation/carnitine transporter (OCTN)
	Placenta
	l-type amino acid transporter (LAT)
発行日	2017-01
収録物名	Reproductive toxicology (Elmsford, N.Y.)
巻(号)	67
ページ	48-55
抄録	Gabapentin (GBP) is a widely used antiepileptic drug, with potential for use in the treatment of epilepsy in pregnant women. Although studies have examined GBP transport mechanisms across the blood-brain barrier, kidney, and intestine, the mechanism in the placenta has not been fully elucidated. We previously reported that GBP accumulates at high concentrations in human placental choriocarcinoma BeWo cells. The purpose of this study was to examine the transport mechanism of GBP in placental choriocarcinoma cells (BeWo and JEG-3), and to identify the carrier involved. High concentrations of intracellular GBP accumulations were also found in JEG-3 cells. A kinetic analysis showed that a single carrier system was involved in the uptake of GBP. Furthermore, substrates for l-type amino acid transporter (LAT) and siRNAs targeted to LAT1 significantly decreased GBP uptake. Our observations from this study suggest that LAT1 is the main contributor to GBP transport in placental choriocarcinoma cells.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	1873-1708
関連情報	PMID 27818298
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.reprotox.2016.11.002