MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment

Hosotani, Marina; Ichii, Osamu; Nakamura, Teppei; Masum, Md Abdul; Otani, Yuki; Otsuka-Kanazawa, Saori; Elewa, Yaser H. A.; Kon, Yasuhiro

MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment

Hosotani, Marina; Ichii, Osamu; Nakamura, Teppei et al.

Reproduction, Fertility and Development, 2019, 31(4), 760-773

Permalink : https://hdl.handle.net/2115/75893

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アクセス権	open access
URI	https://hdl.handle.net/2115/75893
タイトル	MRL/MpJ mice produce more oocytes and exhibit impaired fertilisation and accelerated luteinisation after superovulation treatment
著者	Hosotani, Marina ; Ichii, Osamu ; Nakamura, Teppei ; Masum, Md Abdul ; Otani, Yuki ; Otsuka-Kanazawa, Saori ; Elewa, Yaser H. A. ; Kon, Yasuhiro
著者名 Hosotani, Marina
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
著者名 Nakamura, Teppei
著者名 Masum, Md Abdul
著者名 Otani, Yuki
著者名 Otsuka-Kanazawa, Saori
著者名 Elewa, Yaser H. A.
e-Rad_Researcher 10178402 著者名 Kon, Yasuhiro
言語	英語
キーワード	extracellular matrix
	histology
	invitro fertilisation
	ovary
発行日	2019-03
出版者	CSIRO Publishing
収録物名	Reproduction, Fertility and Development
巻(号)	31(4)
ページ	760-773
抄録	MRL/MpJ mice exhibit distinct phenotypes in several biological processes, including wound healing. Herein we report two unique phenotypes in the female reproductive system of MRL/MpJ mice that affect ovulation and luteinisation. We found that superovulation treatment resulted in the production of significantly more oocytes in MRL/MpJ than C57BL/6 mice (71.0 +/- 13.4 vs 26.8 +/- 2.8 respectively). However, no exon mutations were detected in genes coding for female reproductive hormones or their receptors in MRL/MpJ mice. In addition, the fertilisation rate was lower for ovulated oocytes from MRL/MpJ than C57BL/6 mice, with most of the fertilised oocytes showing abnormal morphology, characterised by deformation and cytolysis. Histological tracing of luteinisation showed that MRL/MpJ mice formed corpora lutea within 36h after ovulation, whereas C57BL/6 mice were still at the corpora haemorrhagica formation stage after 36h. The balance between the expression of matrix metalloproteinases and their tissue inhibitors shifted towards the former earlier after ovulation in MRL/MpJ than C57BL/6 mice. This result indicates a possible link between accelerated extracellular matrix remodelling in the ovulated or ruptured follicles and luteinisation in MRL/MpJ mice. Together, these findings reveal novel phenotypes in MRL/MpJ mice that provide novel insights into reproductive biology.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
PISSN	1031-3613
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1071/RD18319