open access

https://hdl.handle.net/2115/75966

Recessive resistance governed by a major quantitative trait locus restricts clover yellow vein virus in mechanically but not graft-inoculated cultivated soybeans

英語

virus-plant interactions

2019-08

American Phytopathological Society(APS)

Molecular Plant-Microbe Interactions

32(8)

1026-1037

Clover yellow vein virus (ClYVV) infects and causes disease in legume plants. However, here we found that ClYVV isolate No. 30 (ClYVV-No.30) inefficiently multiplied or spread via cell-to-cell movement in mechanically inoculated leaves of a dozen soybean (Glycine max) cultivars, and resulted in failure to spread systemically. Soybean plants also had a similar resistance phenotype against additional ClYVV isolates. In contrast, all but one of 24 tested accessions of wild soybeans (G. soja) were susceptible to ClYVV-No.30. Graft inoculation of cultivated soybean TK780 with ClYVV-No.30-infected wild soybean B01167 scion resulted in systemic infection of the cultivated soybean rootstock. This suggests that upon mechanical inoculation the cultivated soybean inhibits ClYVV-No.30 at infection steps prior to the systemic spread of the virus via vascular systems. Systemic infection of all of F1 plants from crossing between TK780 and B01167 and 68 out of 76 F2 plants with ClYVV-No.30 indicated recessive inheritance of the resistance. Further genetic analysis using 64 recombinant inbred lines between TK780 and B01167 detected one major quantitative trait locus, designated d-cv, for the resistance that was positioned in the linkage group D1b (chromosome 2). The mapped region on soybean genome suggests that d-cv is not an allele of the known resistance genes against soybean mosaic virus.

学術雑誌論文

Accepted Manuscript

Reprinted from Junya Abe, Yongzhi Wang, Tetsuya Yamada, Masako Sato, Takuya Ono, Go Atsumi, Jun Abe, M. R. Hajimorad, and Kenji Suto Nakahara, Aug 2019, Recessive resistance governed by a major quantitative trait locus restricts clover yellow vein virus in mechanically but not graft-inoculated cultivated soybeans, Molecular Plant-Microbe Interactions, 32(8):p1026-1037.

0894-0282

1943-7706

AA10671675