open access

https://hdl.handle.net/2115/75999

Heterogeneous distribution of doublecortin-expressing cells surrounding the rostral migratory stream in the juvenile mouse

英語

RR

2018-11-01

John Wiley & Sons

The journal of comparative neurology

526(16)

2631-2646

In the postnatal mammalian brain, neural stem cells of the ventricular-subventricular zone continue to generate doublecortin (Dcx)-expressing immature neurons. Throughout life, these immature neurons migrate to the olfactory bulb through the rostral migratory stream (RMS). In this study, we investigated the distribution of these putative immature neurons using enhanced green fluorescent protein (EGFP) expression in the area surrounding the RMS of the juvenile Dcx-EGFP mice. Through the combined use of an optical clearing reagent (a 2,2 '-thiodiethanol solution) and two-photon microscopy, we visualized three-dimensionally the EGFP-positive cells in the entire RMS and its surroundings. The resulting wide-field and high-definition images along with computational image processing methods developed in this study were used to comprehensively determine the position of the EGFP-positive cells. Our findings revealed that the EGFP-positive cells were heterogeneously distributed in the area surrounding the RMS. In addition, the orientation patterns of the leading process of these cells, which displayed the morphology of migrating immature neurons, differed depending on their location. These novel results provide highly precise morphological information for immature neurons and suggest that a portion of immature neurons may be detached from the RMS and migrate in various directions.

学術雑誌論文

Accepted Manuscript

This is the peer reviewed version of the following article: Yuka Aoyagi, Terumasa Hibi, Yoshitaka Kimori, Masato Sawada, Ryosuke Kawakami, Kazunobu Sawamoto and Tomomi Nemoto. Heterogeneous distribution of doublecortin‐expressing cells surrounding the rostral migratory stream in the juvenile mouse. The Journal of Comparative Neurology. Volume526, Issue16 (November 1, 2018). Pages 2631-2646, which has been published in final form at https://doi.org/10.1002/cne.24521 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.

0021-9967