An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model

El-naseery, Nesma Ibraheim; Elewa, Yaser Hosny Ali; Ichii, Osamu; Kon, Yasuhiro

An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model

El-naseery, Nesma Ibraheim; Elewa, Yaser Hosny Ali; Ichii, Osamu et al.

Annals of anatomy - Anatomischer anzeiger, 2018, 220, 9-20

Permalink : https://hdl.handle.net/2115/76001

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アクセス権	open access
URI	https://hdl.handle.net/2115/76001
タイトル	An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat model
著者	El-naseery, Nesma Ibraheim ; Elewa, Yaser Hosny Ali ; Ichii, Osamu ; Kon, Yasuhiro
著者名 El-naseery, Nesma Ibraheim
ORCID 0000-0002-7347-4587 著者名 Elewa, Yaser Hosny Ali
e-Rad_Researcher 60547769 著者名 Ichii, Osamu
e-Rad_Researcher 10178402 著者名 Kon, Yasuhiro
言語	英語
キーワード	Parotid gland
	Ovariectomized
	Mesenchymal stromal cells
	Immunohistochemistry
発行日	2018-11
出版者	Elsevier
収録物名	Annals of anatomy - Anatomischer anzeiger
巻(号)	220
ページ	9-20
抄録	The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX + hUCB-MSCs). At 3 months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, alpha-smooth muscle actin (alpha-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, alpha-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX + hUCBMSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration. (C) 2018 Elsevier GmbH. All rights reserved.
資源タイプ	学術雑誌論文
出版タイプ	Accepted Manuscript
権利情報	Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
権利情報	© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
PISSN	0940-9602
関連情報 (isVersionOf)	出版社版DOI https://doi.org/10.1016/j.aanat.2018.06.006