Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice

Islam, Md Atiqul; Torigoe, Daisuke; Kameda, Yayoi et al.

Infection, Genetics and Evolution, 2018, 65, 65-71

Permalink : https://hdl.handle.net/2115/76048

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アクセス権
open access
URI
https://hdl.handle.net/2115/76048
タイトル
Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice
著者
Islam, Md Atiqul ; Torigoe, Daisuke ; Kameda, Yayoi ; Irie, Takao ; Kouguchi, Hirokazu ; Nakao, Ryo ; Masum, Md Abdul ; Ichii, Osamu ; Kon, Yasuhiro ; Tag-EL-Din-Hassan, Hassan T. ; Morimatsu, Masami ; Yagi, Kinpei ; Agui, Takashi
著者名
Islam, Md Atiqul
著者名
Torigoe, Daisuke
著者名
Kameda, Yayoi
著者名
Irie, Takao
e-Rad_Researcher
50435567
著者名
Kouguchi, Hirokazu
e-Rad_Researcher
50633955
著者名
Nakao, Ryo
著者名
Masum, Md Abdul
e-Rad_Researcher
60547769
著者名
Ichii, Osamu
e-Rad_Researcher
10178402
著者名
Kon, Yasuhiro
著者名
Tag-EL-Din-Hassan, Hassan T.
e-Rad_Researcher
70241370
著者名
Morimatsu, Masami
著者名
Yagi, Kinpei
e-Rad_Researcher
00212457
著者名
Agui, Takashi
言語
英語
キーワード
Echinococcus multilocularis
Protoscolex
Congenic mouse
Quantitative trait locus
発行日
2018-11
出版者
Elsevier
収録物名
Infection, Genetics and Evolution
巻(号)
65
ページ
65-71
抄録
The resistance/susceptibility to Echinococcus multilocularis infection in mice is genetically controlled. However, genetic factors responsible for these differences remain unknown. Our previous study in genetic linkage analysis has revealed that there is a significant quantitative trait locus (QTL) for the establishment of cyst (Emcysl), and a highly significant QTL for the development of protoscolex of E. multilocularis larvae (Empscl), on mouse chromosomes 6 and 1, respectively. The current study aimed to confirm these QTLs and narrow down the critical genetic region that controls resistance/susceptibility to E. multilocularis infection by establishing congenic and subcongenic lines from C57BL/6 (B6) and DBA/2 (D2) mice. For protoscolex development phenotype, two congenic lines, B6.D2-Empscl and D2.B6-Empscl were developed, where responsible QTL, Empsc1 was introgressed from D2 into B6 background and vice versa. For cyst establishment phenotype, two congenic lines, B6.D2-Emcys1 and D2.B6-Emcys1 were developed, where responsible QTL, Emcys1 was introgressed from D2 into B6 background and vice versa. Because there was no significant difference in cyst establishment between B6.D2Emcysl and D2.B6-Emcys1 mice after challenge with E. multilocularis, it is suggested that the Emcysl does not solely control the cyst establishment in mouse liver. However, infection experiments with B6.D2-Empscl and D2.B6-Empsc1 mice showed a significant difference in protoscolex development in the cyst. It confirms that the Empsc1 controls phenotype of the protoscolex development in the cyst. Subsequently, two subcongenic lines, B6.D2-Empsc1.1 and B6.D2-Empsc1.2 from B6.D2-Emcysl and one subcongenic line, D2.B6-Empsc1.1 from D2.B6-Empscl were developed to narrow down the critical region responsible for protoscolex development. From the results of infection experiments with E. multilocularis in these subcongenic mice, it is concluded that a gene responsible for protoscolex development is located between D1Mit290 (68.1 cM) and D1Mit511 (97.3 cM).
資源タイプ
学術雑誌論文
出版タイプ
Accepted Manuscript
権利情報
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
PISSN
1567-1348
関連情報
PMID
30030204
関連情報 (isVersionOf)
出版社版DOI
https://doi.org/10.1016/j.meegid.2018.07.017