Identification of novel biomarkers of hepatocellular carcinoma by high‐definition mass spectrometry: Ultrahigh‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry and desorption electrospray ionization mass spectrometry imaging

Nagai, Koshi; Uranbileg, Baasanjav; Chen, Zhen et al.

Rapid Communications in Mass Spectrometry, 2019, 34(S1), e8551

Permalink : https://hdl.handle.net/2115/76132

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アクセス権
open access
URI
https://hdl.handle.net/2115/76132
タイトル
Identification of novel biomarkers of hepatocellular carcinoma by high‐definition mass spectrometry: Ultrahigh‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry and desorption electrospray ionization mass spectrometry imaging
著者
Nagai, Koshi ; Uranbileg, Baasanjav ; Chen, Zhen ; Fujioka, Amane ; Yamazaki, Takahiro ; Matsumoto, Yotaro ; Tsukamoto, Hiroki ; Ikeda, Hitoshi ; Yatomi, Yutaka ; Chiba, Hitoshi ; Hui, Shu‐Ping ; Nakazawa, Toru ; Saito, Ritsumi ; Koshiba, Seizo ; Aoki, Junken ; Saigusa, Daisuke ; Tomioka, Yoshihisa
著者名
Nagai, Koshi
著者名
Uranbileg, Baasanjav
著者名
Chen, Zhen
著者名
Fujioka, Amane
著者名
Yamazaki, Takahiro
著者名
Matsumoto, Yotaro
著者名
Tsukamoto, Hiroki
著者名
Ikeda, Hitoshi
著者名
Yatomi, Yutaka
著者名
Chiba, Hitoshi
e-Rad_Researcher
90337030
著者名
Hui, Shu‐Ping
著者名
Nakazawa, Toru
著者名
Saito, Ritsumi
著者名
Koshiba, Seizo
著者名
Aoki, Junken
著者名
Saigusa, Daisuke
著者名
Tomioka, Yoshihisa
言語
英語
発行日
2019-08-14
出版者
Wiley
収録物名
Rapid Communications in Mass Spectrometry
巻(号)
34(S1)
ページ
e8551
抄録
RATIONALE:Hepatocellular carcinoma (HCC) is a highly malignant disease for which the development of prospective or prognostic biomarkers is urgently required. Although metabolomics is widely used for biomarker discovery, there are some bottlenecks regarding the comprehensiveness of detected features, reproducibility of methods, and identification of metabolites. In addition, information on localization of metabolites in tumor tissue is needed for functional analysis. Here, we developed a wide-polarity global metabolomics (G-Met) method, identified HCC biomarkers in human liver samples by high-definition mass spectrometry (HDMS), and demonstrated localization in cryosections using desorption electrospray ionization MS imaging (DESI-MSI) analysis. METHODS:Metabolic profiling of tumor (n = 38) and nontumor (n = 72) regions in human livers of HCC was performed by an ultrahigh-performance liquid chromatography quadrupole time-of-flight MS (UHPLC/QTOFMS) instrument equipped with a mixed-mode column. The HCC biomarker candidates were extracted by multivariate analyses and identified by matching values of the collision cross section and their fragment ions on the mass spectra obtained by HDMS. Cryosections of HCC livers, which included both tumor and nontumor regions, were analyzed by DESI-MSI. RESULTS:From the multivariate analysis, m/z 904.83 and m/z 874.79 were significantly high and low, respectively, in tumor samples and were identified as triglyceride (TG) 16:0/18:1(9Z)/20:1(11Z) and TG 16:0/18:1(9Z)/18:2(9Z,12Z) using the synthetic compounds. The TGs were clearly localized in the tumor or nontumor areas of the cryosection. CONCLUSIONS:Novel biomarkers for HCC were identified by a comprehensive and reproducible G-Met method with HDMS using a mixed-mode column. The combination analysis of UHPLC/QTOFMS and DESI-MSI revealed that the different molecular species of TGs were associated with tumor distribution and were useful for characterizing the progression of tumor cells and discovering prospective biomarkers.
資源タイプ
学術雑誌論文
出版タイプ
Version of Record
権利情報
Creative Commons Attribution 4.0 International
PISSN
0951-4198
EISSN
1097-0231
関連情報
PMID
31412144
関連情報 (isIdenticalTo)
出版社版DOI
https://doi.org/10.1002/rcm.8551