Plasma-free amino acid profiles in dogs with hepatocellular carcinoma

Leela-arporn, Rommaneeya; Ohta, Hiroshi; Tamura, Masahiro; Nagata, Noriyuki; Sasaoka, Kazuyoshi; Dermlim, Angkhana; Nisa, Khoirun; Osuga, Tatsuyuki; Morishita, Keitaro; Sasaki, Noboru; Takiguchi, Mitsuyoshi

Plasma-free amino acid profiles in dogs with hepatocellular carcinoma

Leela-arporn, Rommaneeya; Ohta, Hiroshi; Tamura, Masahiro et al.

Journal of Veterinary Internal Medicine, 2019, 33(4), 1653-1659

Permalink : https://hdl.handle.net/2115/76135

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https://doi.org/10.1111/jvim.15512			22

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アクセス権	open access
URI	https://hdl.handle.net/2115/76135
タイトル	Plasma-free amino acid profiles in dogs with hepatocellular carcinoma
著者	Leela-arporn, Rommaneeya ; Ohta, Hiroshi ; Tamura, Masahiro ; Nagata, Noriyuki ; Sasaoka, Kazuyoshi ; Dermlim, Angkhana ; Nisa, Khoirun ; Osuga, Tatsuyuki ; Morishita, Keitaro ; Sasaki, Noboru ; Takiguchi, Mitsuyoshi
著者名 Leela-arporn, Rommaneeya
著者名 Ohta, Hiroshi
著者名 Tamura, Masahiro
著者名 Nagata, Noriyuki
著者名 Sasaoka, Kazuyoshi
著者名 Dermlim, Angkhana
著者名 Nisa, Khoirun
著者名 Osuga, Tatsuyuki
著者名 Morishita, Keitaro
著者名 Sasaki, Noboru
e-Rad_Researcher 70261336 著者名 Takiguchi, Mitsuyoshi
言語	英語
キーワード	biomarker
	dog
	liver tumor
	metabolomics
発行日	2019-07
出版者	John Wiley & Sons
収録物名	Journal of Veterinary Internal Medicine
巻(号)	33(4)
ページ	1653-1659
抄録	Background Metabolomic analysis using blood samples has been suggested to be useful for the early detection of cancer. Among metabolites, plasma-free amino acid (PFAA) profiles are potential diagnostic biomarkers for several diseases including cancer. However, the relationship between PFAA concentrations and liver tumors in dogs remains unknown. Objective To determine the characteristics of PFAA profiles of dogs with hepatocellular carcinoma (HCC) and correlated clinical features. Animals Thirty-four client-owned dogs diagnosed with HCC (n = 26) and benign liver diseases (n = 8) and 11 age-matched healthy dogs. Methods Prospective study using heparinized blood samples from fasted dogs. Plasma was deproteinized, and the concentrations of 21 amino acids were measured using an automated high-performance liquid chromatography amino acid analyzer. Results Plasma glutamic acid concentrations were significantly different among groups (P < .0024 after Bonferroni correction). Compared to healthy dogs, dogs with HCC and benign liver diseases had significantly higher concentrations of glutamic acid by post hoc analysis. However, no significant difference in the PFAA profiles of HCC and benign liver diseases were detected. In addition, preoperative and postoperative PFAA profiles of dogs with HCC were not significantly different. Conclusions and Clinical Importance Increased glutamic acid concentrations might play a role in the development or be a consequence of liver tumor formation. However, PFAA profiles of HCC could not be differentiated from those of benign lesions. In addition, glutamic acid concentrations did not change after surgical resection. These results indicate that PFAA profiles may not be useful biomarkers for detecting HCC in dogs.
資源タイプ	学術雑誌論文
出版タイプ	Version of Record
権利情報	Creative Commons Attribution-NonCommercial 4.0 International
PISSN	0891-6640
関連情報 (isIdenticalTo)	出版社版DOI https://doi.org/10.1111/jvim.15512